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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents



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ID: 332614.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
The vacuolar transporter chaperone (VTC) complex is required for microautophagy.
Authors:Uttenweiler, A.; Schwarz, Heinz; Neumann, H.; Mayer, A.
Language:English
Date of Publication (YYYY-MM-DD):2007-01
Title of Journal:Mol Biol Cell.
Volume:18
Issue / Number:1
Start Page:166
End Page:175
Sequence Number of Article:17079729
Review Status:not specified
Audience:Not Specified
Abstract / Description:Microautophagy involves direct invagination and fission of the vacuolar/lysosomal membrane under nutrient limitation. This occurs by an autophagic tube, a specialized vacuolar membrane invagination that pinches off vesicles into the vacuolar lumen. In this study we have identified the VTC (vacuolar transporter chaperone) complex as required for microautophagy. The VTC complex is present on the ER and vacuoles and at the cell periphery. On induction of autophagy by nutrient limitation the VTC complex is recruited to and concentrated on vacuoles. The VTC complex is inhomogeneously distributed within the vacuolar membranes, showing an enrichment on autophagic tubes. Deletion of the VTC complex blocks microautophagic uptake into vacuoles. The mutants still form autophagic tubes but the production of microautophagic vesicles from their tips is impaired. In line with this, affinity-purified antibodies to the Vtc proteins inhibit microautophagic uptake in a reconstituted system in vitro. Our data suggest that the VTC complex is an important constituent of autophagic tubes and that it is required for scission of microautophagic vesicles from these tubes.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen
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