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          Institute: Max-Planck-Arbeitsgruppen für strukturelle Molekularbiologie     Collection: Arbeitsgruppe Zytoskelett     Display Documents

ID: 333616.0, Max-Planck-Arbeitsgruppen für strukturelle Molekularbiologie / Arbeitsgruppe Zytoskelett
The beta-propensity of Tau determines aggregation and synaptic loss in inducible mouse models of tauopathy
Authors:Eckermann, K.; Mocanu, M. M.; Khlistunova, I.; Biernat, J.; Nissen, A.; Hofmann, A.; Schonig, K.; Bujard, H.; Haemisch, A.; Mandelkow, E.; Zhou, L.; Rune, G.; Mandelkow, E. M.
Date of Publication (YYYY-MM-DD):2007-10-26
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J Biol Chem
Issue / Number:43
Start Page:31755
End Page:31765
Review Status:not specified
Audience:Not Specified
Abstract / Description:Neurofibrillary lesions are characteristic for a group of human diseases, named tauopathies, which are characterized by prominent intracellular accumulations of abnormal filaments formed by the microtubule-associated protein Tau. The tauopathies are accompanied by abnormal changes in Tau protein, including pathological conformation, somatodendritic mislocalization, hyperphosphorylation, and aggregation, whose interdependence is not well understood. To address these issues we have created transgenic mouse lines in which different variants of full-length Tau are expressed in a regulatable fashion, allowing one to switch the expression on and off at defined time points. The Tau variants differ by small mutations in the hexapeptide motifs that control the ability of Tau to adopt a beta-structure conformation and hence to aggregate. The "pro-aggregation" mutant DeltaK280, derived from one of the mutations observed in frontotemporal dementias, aggregates avidly in vitro, whereas the "anti-aggregation" mutant DeltaK280/PP cannot aggregate because of two beta-breaking prolines. In the transgenic mice, the pro-aggregation Tau induces a pathological conformation and pre-tangle aggregation, even at low expression levels, the anti-aggregation mutant does not. This illustrates that abnormal aggregation is primarily controlled by the molecular structure of Tau in vitro and in the organism. Both variants of Tau become mislocalized and hyperphosphorylated independently of aggregation, suggesting that localization and phosphorylation are mainly a consequence of increased concentration. These pathological changes are reversible when the expression of Tau is switched off. The pro-aggregation Tau causes a strong reduction in spine synapses.
Free Keywords:Amino Acid Motifs; Animals; *Disease Models, Animal; Humans; Immunohistochemistry; Mice; Mice, Transgenic; Mutation; Phosphorylation; Protein Conformation; Protein Isoforms; Synapses/*pathology; Tauopathies/genetics/metabolism/*pathology; tau Proteins/*chemistry/genetics/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:Max-Planck-Arbeitsgruppen für strukturelle Molekularbiologie/Zytoskelett
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