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          Institute: Max-Planck-Arbeitsgruppen für strukturelle Molekularbiologie     Collection: Arbeitsgruppe Zytoskelett     Display Documents

ID: 333632.0, Max-Planck-Arbeitsgruppen für strukturelle Molekularbiologie / Arbeitsgruppe Zytoskelett
Structural and microtubule binding properties of tau mutants of frontotemporal dementias
Authors:Fischer, D.; Mukrasch, M. D.; von Bergen, M.; Klos-Witkowska, A.; Biernat, J.; Griesinger, C.; Mandelkow, E.; Zweckstetter, M.
Date of Publication (YYYY-MM-DD):2007-03-13
Title of Journal:Biochemistry
Journal Abbrev.:Biochemistry
Issue / Number:10
Start Page:2574
End Page:2582
Review Status:not specified
Audience:Not Specified
Abstract / Description:Several mutations in the gene encoding the microtubule-associated protein tau are responsible for the formation of neurofibrillary inclusions in frontotemporal dementia with Parkinsonism linked to chromosome 17 (FTDP-17). Here we present the high-resolution characterization of the conformational properties of two FTDP-17 mutants of the four-repeat domain of tau, P301L and DeltaK280, and their properties for binding to polyanions and microtubules. Multidimensional NMR spectroscopy shows that the mutations do no lead to a significant increase in the level of beta-structure in their monomeric state, even though the mutations strongly promote beta-structure during aggregation. However, local structural changes are induced in the second repeat. These changes only weakly affect the binding to the polyanion heparin, which promotes paired helical filament formation. The extent of binding to microtubules, however, is strongly decreased. Our results demonstrate that the reversible binding of tau to microtubules involves specific interactions, which are not essential for binding to polyanions.
Free Keywords:Dementia/genetics/*metabolism; Humans; Microtubule-Associated Proteins/metabolism; Microtubules/drug effects/*metabolism; Mutation; Polymers/metabolism; Protein Structure, Secondary; Sodium Chloride/pharmacology; tau Proteins/chemistry/*genetics/*metabolism
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:Max-Planck-Arbeitsgruppen für strukturelle Molekularbiologie/Zytoskelett
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