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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 334035.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Conserved molecular portraits of bovine and human blastocysts
as a consequence of the transition from maternal to embryonic
control of gene expression
Authors:Adjaye, James; Herwig, Ralf; Brink, Thore C.; Herrmann, Doris; Greber, Boris; Sudheer, Smita; Groth, Detlef; Carnwath, Joseph W.; Lehrach, Hans; Niermann, Heiner
Date of Publication (YYYY-MM-DD):2007-10-22
Title of Journal:Physiological Genomics
Journal Abbrev.:Physiol Genomics
Issue / Number:2
Start Page:315
End Page:327
Copyright:© 2007 by the American Physiological Society
Review Status:not specified
Audience:Experts Only
Abstract / Description:The present study investigated mRNA expression profiles of bovine oocytes and blastocysts by using a cross-species hybridization approach employing an array consisting of 15,529 human cDNAs as probe, thus enabling the identification of conserved genes during human and bovine preimplantation development. Our analysis revealed 419 genes that were expressed in both oocytes and blastocysts. The expression of 1,324 genes was detected exclusively in the blastocyst, in contrast to 164 in the oocyte including a significant number of novel genes. Genes indicative for transcriptional and translational control (ELAVL4, TACC3) were overexpressed in the oocyte, whereas cellular trafficking (SLC2A14, SLC1A3), proteasome (PSMA1, PSMB3), cell cycle (BUB3, CCNE1, GSPT1), and protein modification and turnover (TNK1, UBE3A) genes were found to be overexpressed in blastocysts. Transcripts implicated in chromatin remodeling were found in both oocytes (NASP, SMARCA2) and blastocysts (H2AFY, HDAC7A). The trophectodermal markers PSG2 and KRT18 were enriched 5- and 50-fold in the blastocyst. Pathway analysis revealed differential expression of genes involved in 107 distinct signaling and metabolic pathways. For example, phosphatidylinositol signaling and gluconeogenesis were prominent pathways identified in the blastocyst. Expression patterns in bovine and human blastocysts were to a large extent identical. This analysis compared the transcriptomes of bovine oocytes and blastocysts and provides a solid foundation for future studies on the first major differentiation events in blastocysts and identification of a set of markers indicative for regular mammalian development.
Free Keywords:Oocytes; Microarrays; Cross-species hybridization
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Biotechnology, Institute for Animal Science, Neustadt, Germany
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