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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 334055.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Nodal signaling activates differentiation genes during zebrafish gastrulation
Authors:Bennett, James D.; Joubin, Katherine; Cheng, Simon; Aanstad, Pia; Herwig, Ralf; Clark, Matthew; Lehrach, Hans; Schier, Alexander F.
Date of Publication (YYYY-MM-DD):2007-04-15
Title of Journal:Developmental Biology
Journal Abbrev.:Dev Biol
Issue / Number:2
Start Page:525
End Page:540
Copyright:© 2008 Elsevier Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Nodal signals induce mesodermal and endodermal progenitors during vertebrate development. To determine the role of Nodal signaling at a genomic level, we isolated Nodal-regulated genes by expression profiling using macroarrays and gene expression databases. Putative Nodal-regulated genes were validated by in situ hybridization screening in wild type and Nodal signaling mutants. 46 genes were identified, raising the currently known number of Nodal-regulated genes to 72. Based on their expression patterns along the dorsoventral axis, most of these genes can be classified into two groups. One group is expressed in the dorsal margin, whereas the other group is expressed throughout the margin. In addition to transcription factors and signaling components, the screens identified several new functional classes of Nodal-regulated genes, including cytoskeletal components and molecules involved in protein secretion or endoplasmic reticulum stress. We found that x-box binding protein-1 (xbp1) is a direct target of Nodal signaling and required for the terminal differentiation of the hatching gland, a specialized secretory organ whose specification is also dependent on Nodal signaling. These results indicate that Nodal signaling regulates not only specification genes but also differentiation genes.
Free Keywords:Nodal; Zebrafish; Mesoderm; Endoderm; Gastrulation; Xbp1; Terminal differentiation; Hatching gland; Secretion
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Developmental Genetics Program, Skirball Institute of Biomolecular Medicine, and Department of Cell Biology, USA;
Department of Radiation Oncology New York University School of Medicine, New York, NY 10016, USA;
Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridge, CB10 1SA, UK;
Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143, USA;
Department of Molecular and Cellular Biology, Harvard Stem Cell Institute, Center for Brain Science, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA
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