Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Genetik     Collection: Department of Human Molecular Genetics     Display Documents



  history
ID: 334813.0, MPI für molekulare Genetik / Department of Human Molecular Genetics
Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1.
Authors:Winter, Jennifer; Kunath, Melanie; Roepcke, Stefan; Krause, Sven; Schneider, Rainer; Schweiger, Susann
Language:English
Date of Publication (YYYY-MM-DD):2007-11-15
Title of Journal:BMC Molecular Biology [Elektronische Ressource]
Volume:8
Issue / Number:1
Start Page:105
End Page:133
Copyright:© 1999-2008 BioMed Central Ltd unless otherwise stated
Review Status:not specified
Audience:Experts Only
Abstract / Description:Background
Mutations in the X-linked MID1 gene are responsible for Opitz G/BBB syndrome, a malformation disorder of developing midline structures. Previous Northern blot analyses revealed the existence of at least three MID1 transcripts of differing lengths.
Results
Here we show that alternative polyadenylation generates the size differences observed in the Northern blot analyses. Analysis of EST data together with additional Northern blot analyses proved tissue-specific usage of the alternative polyadenylation sites. Bioinformatic characterization of the different 3'UTRs of MID1 revealed numerous RNA-protein interaction motifs, several of which turned out to be conserved between different species. Furthermore, our data suggest that mRNA termination at different polyadenylation sites is predetermined by the choice of alternative 5'UTRs and promoters of the MID1 gene, a mechanism that efficiently allows synergistic function of 5' and 3'UTRs.
Conclusions
MID1 expression is tightly regulated through concerted action of alternative promoters and alternative polyadenylation signals both during embryonic development and in the adult.
External Publication Status:published
Document Type:Article
Communicated by:Hans-Hilger Ropers
Affiliations:MPI für molekulare Genetik
Identifiers:ISSN:1471-2199
DOI:10.1186/1471-2199-8-105
URL:http://www.biomedcentral.com/content/pdf/1471-2199...
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.