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          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Systems Biology     Display Documents

ID: 337096.0, MPI für Dynamik komplexer technischer Systeme / Systems Biology
Profiling of external metabolites during production of hantavirus nucleocapsid protein with recombinant Saccharomyces cerevisiae
Authors:Antoniukas, L.; Grammel, H.; Sasnauskas, K.; Reichl, U.
Date of Publication (YYYY-MM-DD):2008
Title of Journal:Biotechnology Letters
Issue / Number:3
Start Page:415
End Page:420
Review Status:not specified
Audience:Experts Only
Abstract / Description:Recombinant strains of Saccharomyces cerevisiae, producing hantavirus Puumala nucleocapsid protein for diagnostics and as a candidate vaccine were analyzed for uptake and excretion of intermediary metabolites during process optimization studies of fed-batch bioreactor cultures. Concentrations of glucose, maltose, galactose, pyruvate, acetaldehyde, ethanol, acetate, succinate and formaldehyde (used as a selection agent) were measured in the culture medium in order to find a metabolite pattern, indicative for the physiological state of the producer culture. When the inducer galactose was employed as a growth substrate, the metabolite profile of recombinant yeast cells was different from those of the non-recombinant original strain which excreted considerable amounts of metabolites with this substrate. In contrast, galactose-induced heterologous gene expression was indicated by the absence of excreted intermediary metabolites, except succinate. A model strain expressing a GFP fusion of hantavirus nucleocapsid protein differed in the excretion of metabolites from strains without GFP. In addition, the influence of alkali ions, employed for pH control is also demonstrated.
© Springer Science+Business Media B.V. 2007
[accessed June 6, 2008]
External Publication Status:published
Document Type:Article
Communicated by:Ernst-Dieter Gilles
Affiliations:MPI für Dynamik komplexer technischer Systeme/Systems Biology
MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
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