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          Institute: MPI für Evolutionsbiologie     Collection: Ecophysiology     Display Documents

ID: 337259.0, MPI für Evolutionsbiologie / Ecophysiology
Genetic diversity of cyanobacterial communities in Lake Kinneret (Israel) using 16S rRNA gene, psbA and ntcA sequence analyses
Authors:Junier, Pilar; Witzel, Karl-Paul; Hadas, Ora
Date of Publication (YYYY-MM-DD):2007-11-29
Title of Journal:Aquatic Microbial Ecology
Journal Abbrev.:Aquat Microb Ecol
Issue / Number:3
Start Page:233
End Page:241
Review Status:not specified
Audience:Not Specified
Abstract / Description:The genetic diversity of cyanobacterial communities was studied at various depths in Lake Kinneret (Israel). Denaturing gradient gel electrophoresis (DGGE) of specific 16S rRNA gene PCR products showed significant differences in the cyanobacterial community structure between epi- and hypolimnetic waters. Sequences of clone libraries prepared from 16S rRNA gene PCR products from epi- and,hypolimnion revealed the presence of at least 11 different groups of cyanobacteria. Clones related to the unicellular cyanobacteria (Chroococcales and picocyanobacteria) dominated the clone libraries from both depths. New primers to amplify the gene coding for the photosystem 11 reaction centre (psbA) and the nitrogen regulator gene (ntcA) of cyanobacteria were developed and used for further characterization of the cyanobacterial communities from the lake. Sequences of psbA amplicons clustered with those from 2 different groups of marine Synechococcus and Chroococcales. Cloned ntcA amplicons from the lake were closely related and did not cluster with sequences from cultured cyanobacteria or other environmental sequences from this gene. All the molecular markers analyzed here showed similarity to sequences from some groups of cyanobacteria in the lake and those so far found in marine habitats.
Free Keywords:gloeocapsa; picophytoplankton; 16S rRNA gene; psbA; ntcA
External Publication Status:published
Document Type:Article
Affiliations:MPI für Limnologie/Abt. Ökophysiologie
External Affiliations:Ecole Polytech Fed Lausanne, CH-1015 Lausanne, Switzerland;
Israel Oceanog & Limnol Res, Kinneret Limnol Lab, IL-14950 Migdal, Israel
Identifiers:ISSN:0948-3055 [ID-No:1]
DOI:10.3354/ame01161 [ID-No:2]
LOCALID:2591/S 38756 [Listen-Nummer/S-Nummer]
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