MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2007 |
|NDEL1 Phosphorylation by Aurora-A Kinase Is Essential for Centrosomal Maturation, Separation, and TACC3 Recruitment|
|Authors:||Mori, Daisuke; Yano, Yoshihisa; Toyo-oka, Kazuhito; Yoshida, Noriyuki; Yamada, Masami; Muramatsu, Masami; Zhang, Dongwei; Saya, Hideyuki; Toyoshima, Yoko Y.; Kinoshita, Kazuhisa; Wynshaw-Boris, Anthony; Hirotsune, Shinji|
|Date of Publication (YYYY-MM-DD):||2007|
|Title of Journal:||Molecular and Cellular Biology|
|Issue / Number:||1|
|Intended Educational Use:||No|
|Abstract / Description:||NDEL1 is a binding partner of LIS1 that participates in the regulation of cytoplasmic dynein function and|
microtubule organization during mitotic cell division and neuronal migration. NDEL1 preferentially localizes
to the centrosome and is a likely target for cell cycle-activated kinases, including CDK1. In particular, NDEL1
phosphorylation by CDK1 facilitates katanin p60 recruitment to the centrosome and triggers microtubule
remodeling. Here, we show that Aurora-A phosphorylates NDEL1 at Ser251 at the beginning of mitotic entry.
Interestingly, NDEL1 phosphorylated by Aurora-A was rapidly downregulated thereafter by ubiquitinationmediated
protein degradation. In addition, NDEL1 is required for centrosome targeting of TACC3 through the
interaction with TACC3. The expression of Aurora-A phosphorylation-mimetic mutants of NDEL1 efficiently
rescued the defects of centrosomal maturation and separation which are characteristic of Aurora-A-depleted
cells. Our findings suggest that Aurora-A-mediated phosphorylation of NDEL1 is essential for centrosomal
separation and centrosomal maturation and for mitotic entry.
|External Publication Status:||published|
|Communicated by:||n. n.|
|Affiliations:||MPI für molekulare Zellbiologie und Genetik|
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