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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2007     Display Documents

ID: 348535.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2007
Straight GDP-tubulin protofilaments form in the presence of taxol
Authors:Elie-Caille, Celine; Severin, Fedor; Helenius, Jonne; Howard, Jonathon; Muller, Daniel J.; Hyman, Anthony A.
Date of Publication (YYYY-MM-DD):2007
Title of Journal:Current Biology
Issue / Number:20
Start Page:1765
End Page:1770
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Microtubules exist in dynamic equilibrium, growing and shrinking by the addition or loss of tubulin dimers from the ends of protofilaments. The hydrolysis of GTP in beta-tubulin destabilizes the microtubule lattice by increasing the curvature of protofilaments in the microtubule and putting strain on the lattice. The observation that protofilament curvature depends on GTP hydrolysis suggests that microtubule destabilizers and stabilizers work by modulating the curvature of the microtubule lattice itself. Indeed, the microtubule destabilizer MCAK has been shown to increase the curvature of protofilaments during depolymerization. Here, we show that the atomic force microscopy (AFM) of individual tubulin protofilaments provides sufficient resolution to allow the imaging of single protofilaments in their native environment. By using this assay, we confirm previous results for the effects of GTP hydrolysis and MCAK on the conformation of protofilaments. We go on to show that taxol stabilizes microtubules by straightening the GDP protofilament and slowing down the transition of protofilaments from straight to a curved configuration.
External Publication Status:published
Document Type:Article
Communicated by:n. n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
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