Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2007     Display Documents



ID: 348602.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2007
Tandem affinity purification of functional TAP-tagged proteins from human cells
Authors:Gregan, Juraj; Riedel, Christian G.; Petronczki, Mark; Cipak, Lubos; Rumpf, Cornelia; Poser, Ina; Buchholz, Frank; Mechtler, Karl; Nasmyth, Kim
Date of Publication (YYYY-MM-DD):2007
Title of Journal:Nature Protocols
Volume:2
Issue / Number:5
Start Page:1145
End Page:1151
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to test the functionality of the Sgo1-TAP protein by RNA interference-mediated depletion of the endogenous human Sgo1. Here, we present an optimized protocol for purification of TAP-tagged Sgo1 protein as well as KIAA1387 from HeLa cells with detailed instructions. The purification protocol can be completed in 1 day and it should be applicable to other proteins.
External Publication Status:published
Document Type:Article
Communicated by:n. n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:834
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.