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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2007     Display Documents

ID: 348611.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2007
RUNX1 DNA-Binding Mutants, Associated with Minimally Differentiated Acute Myelogenous Leukemia, Disrupt Myeloid Differentiation
Authors:Cammenga, Jorg; Niebuhr, Birte; Horn, Stefan; Bergholz, Ulla; Putz, Gabriele; Buchholz, Frank; Lohler, Jurgen; Stocking, Carol
Date of Publication (YYYY-MM-DD):2007
Title of Journal:Cancer Research
Issue / Number:2
Start Page:537
End Page:545
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Mutations in the RUNX1 gene are found at high frequencies in minimally differentiated acute myelogenous leukemia. In addition to null mutations, many of the mutations generate Runx1 DNA-binding (RDB) mutants. To determine if these mutants antagonize wild-type protein activity, cDNAs were transduced into murine bone marrow or human cord blood cells using retroviral vectors. Significantly, the RDB mutants did not act in a transdominant fashion in vivo to disrupt Runx1 activity in either T-cell or platelet development, which are highly sensitive to Runx1 dosage. However, RDB mutant expression impaired expansion and differentiation of the erythroid compartment in which Runx1 expression is normally down-regulated, showing that a RDB-independent function is incompatible with erythroid differentiation. Significantly, both bone marrow progenitors expressing RDB mutants or deficient for Runx1 showed increased replating efficiencies in vitro, accompanied by the accumulation of myeloblasts and dysplastic progenitors, but the effect was more pronounced in RDB cultures. Disruption of the interface that binds CBFbeta, an important cofactor of Runx1, did not impair RDB mutant replating activity, arguing against inactivation of Runx1 function by CBFbeta sequestration. We propose that RDB mutants antagonize Runx1 function in early progenitors by disrupting a critical balance between DNA-binding-independent and DNA-binding-dependent signaling. [Cancer Res 2007;67(2):537-45].
External Publication Status:published
Document Type:Article
Communicated by:n. n.
Affiliations:MPI für molekulare Zellbiologie und Genetik
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