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          Institute: MPI für molekulare Biomedizin     Collection: Publikationen molekulare Biomedizin     Display Documents



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ID: 35096.0, MPI für molekulare Biomedizin / Publikationen molekulare Biomedizin
A transmembrane tight junction protein selectively expressed on endothelial cells and platelets
Authors:Nasdala, I.; Wolburg-Buchholz, K.; Wolburg, H.; Kuhn, A.; Ebnet, K.; Brachtendorf, G.; Samulowitz, U.; Kuster, B.; Engelhardt, B.; Vestweber, D.; Butz, S.
Language:English
Date of Publication (YYYY-MM-DD):2002-05-03
Title of Journal:J Biol Chem
Volume:277
Issue / Number:18
Start Page:16294
End Page:16303
Review Status:not specified
Audience:Not Specified
Abstract / Description:Searching for cell surface proteins expressed at interendothelial cell contacts, we have raised monoclonal antibodies against intact mouse endothelial cells. We obtained two monoclonal antibodies, 1G8 and 4C10, that stain endothelial cell contacts and recognize a protein of 55 kDa. Purification and identification by mass spectrometry of this protein revealed that it contains two extracellular Ig domains, reminiscent of the JAM family, but a much longer 120-amino acid cytoplasmic domain. The antigen is exclusively expressed on endothelial cells of various organs as was analyzed by immunohistochemistry. Immunogold labeling of ultrathin sections of brain as well as skeletal muscle revealed that the antigen strictly colocalizes in capillaries with the tight junction markers occludin, claudin-5, and ZO-1. Upon transfection into MDCK cells, the antigen was restricted to the most apical tip of the lateral cell surface, where it colocalized with ZO-1 but not with beta-catenin. In contrast to JAM-1, however, the 1G8 antigen does not associate with the PDZ domain proteins ZO-1, AF-6, or ASIP/PAR-3, despite the presence of a PDZ-binding motif. The 1G8 antigen was not detected on peripheral blood mouse leukocytes, whereas similar to JAM-1 it was strongly expressed on platelets and megakaryocytes. The 1G8 antigen supports homophilic interactions on transfected Chinese hamster ovary cells. Based on the similarity to the JAM molecules, it is plausible that the 1G8 antigen might be involved in interendothelial cell adhesion.
Free Keywords:Animal
; Antibodies
; Antibodies, Monoclonal
; Blood Platelets/*metabolism
; Brain/metabolism
; Cell Adhesion Molecules/analysis/*physiology
; Endothelium, Vascular/*metabolism
; Megakaryocytes/*metabolism/ultrastructure
; Membrane Proteins/blood/genetics/*metabolism
; Mice
; Microscopy, Immunoelectron
; Muscle, Skeletal/metabolism
; Rabbits
; Rats
; Recombinant Fusion Proteins/metabolism
; Spectrum Analysis, Mass
; Support, Non-U.S. Gov't
; Tight Junctions/*physiology
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Biomedizin
Identifiers:URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:1]
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