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          Institute: MPI für Biochemie     Collection: Structure Research (R. Huber)     Display Documents



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ID: 35336.0, MPI für Biochemie / Structure Research (R. Huber)
Crystallization and preliminary X-ray analysis of the membrane-bound cytochrome c nitrite reductase complex (NrfHA) from Wolinella succinogenes
Authors:Einsle, O.; Stach, P.; Messerschmidt, A.; Klimmek, O.; Simon, J.; Kröger, A.; Kroneck, P. M. H.
Language:English
Date of Publication (YYYY-MM-DD):2002-02
Title of Journal:Acta Crystallographica Section D - Biological Crystallography
Journal Abbrev.:Acta Crystallogr. Sect. D-Biol. Crystallogr.
Volume:58
Start Page:341
End Page:342
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Crystals of the complex between the enzyme cytochrome c nitrite reductase (NrfA) and the membrane-bound quinol oxidase and electron carrier NrfH were grown by vapour diffusion using ammonium sulfate as a precipitant. In the epsilon- proteobacterium Wolinella succinogenes, NrfA and NrfH form a functional membrane-bound complex which catalyzes the last step in the metabolic pathway of nitrate dissimilation. NrfH represents a prototype of a large family of putative bacterial quinol oxidases, the NapC/NirT family, which have been proposed to serve as electron donors for a variety of reductases. Crystal growth of the NrfHA complex was strongly dependent on the presence of detergent; the crystals grown belonged to space group I422.
Comment of the Author/Creator:Date: 2002, FEB
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Structure Research (Huber)/Research Group A. Messerschmidt
External Affiliations:Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, Fach; M665, D-78457 Constance, Germany; Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, D-78457 Constance, Germany; Univ Frankfurt, Inst Mikrobiol, D-60439 Frankfurt, Germany
Identifiers:ISI:000173442200029 [ID No:1]
ISSN:0907-4449 [ID No:2]
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