Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Biochemie     Collection: Structure Research (R. Huber)     Display Documents



  history
ID: 35337.0, MPI für Biochemie / Structure Research (R. Huber)
Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici
Authors:Abt, D. J.; Einsle, O.; Niessen, H.; Krieger, R.; Messerschmidt, A.; Schink, B.; Kroneck, P. M. H.
Language:English
Date of Publication (YYYY-MM-DD):2002-02
Title of Journal:Acta Crystallographica Section D - Biological Crystallography
Journal Abbrev.:Acta Crystallogr. Sect. D-Biol. Crystallogr.
Volume:58
Start Page:343
End Page:345
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Crystals of the molybdo-/iron-sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N-2/H-2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water. Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 Angstrom.
Comment of the Author/Creator:Date: 2002, FEB
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Structure Research (Huber)/Research Group A. Messerschmidt
External Affiliations:Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt,; Postfach M665, D-78457 Constance, Germany; Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, D-78457 Constance, Germany
Identifiers:ISI:000173442200030 [ID No:1]
ISSN:0907-4449 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.