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ID:
35337.0,
MPI für Biochemie / Structure Research (R. Huber) |
Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici |
Authors: | Abt, D. J.; Einsle, O.; Niessen, H.; Krieger, R.; Messerschmidt, A.; Schink, B.; Kroneck, P. M. H. | Language: | English | Date of Publication (YYYY-MM-DD): | 2002-02 | Title of Journal: | Acta Crystallographica Section D - Biological Crystallography | Journal Abbrev.: | Acta Crystallogr. Sect. D-Biol. Crystallogr. | Volume: | 58 | Start Page: | 343 | End Page: | 345 | Review Status: | Peer-review | Audience: | Not Specified | Abstract / Description: | Crystals of the molybdo-/iron-sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N-2/H-2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water. Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 Angstrom. | Comment of the Author/Creator: | Date: 2002, FEB | External Publication Status: | published | Document Type: | Article |
Communicated by: | N.N. | Affiliations: | MPI für Biochemie/Structure Research (Huber)/Research Group A. Messerschmidt
| External Affiliations: | Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt,; Postfach M665, D-78457 Constance, Germany; Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, D-78457 Constance, Germany
| Identifiers: | ISI:000173442200030 [ID No:1] ISSN:0907-4449 [ID No:2] | |
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