Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Biochemie     Collection: Structure Research (R. Huber)     Display Documents



  history
ID: 35337.0, MPI für Biochemie / Structure Research (R. Huber)
Crystallization and preliminary X-ray analysis of the molybdenum-dependent pyrogallol-phloroglucinol transhydroxylase of Pelobacter acidigallici
Authors:Abt, D. J.; Einsle, O.; Niessen, H.; Krieger, R.; Messerschmidt, A.; Schink, B.; Kroneck, P. M. H.
Language:English
Date of Publication (YYYY-MM-DD):2002-02
Title of Journal:Acta Crystallographica Section D - Biological Crystallography
Journal Abbrev.:Acta Crystallogr. Sect. D-Biol. Crystallogr.
Volume:58
Start Page:343
End Page:345
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Crystals of the molybdo-/iron-sulfur protein pyrogallol:phloroglucinol hydroxyltransferase (transhydroxylase; EC 1.97.1.2) from Pelobacter acidigallici were grown by vapour diffusion in an N-2/H-2 atmosphere using polyethylene glycol as a precipitant. In this microorganism, transhydroxylase converts pyrogallol to phloroglucinol in a unique reaction without oxygen transfer from water. Growth of crystals suitable for X-ray analysis was strongly dependent on the presence of dithionite as a reducing agent. The crystals belonged to space group P1 and MAD data were collected on the iron K edge to resolutions higher than 2.5 Angstrom.
Comment of the Author/Creator:Date: 2002, FEB
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Structure Research (Huber)/Research Group A. Messerschmidt
External Affiliations:Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt,; Postfach M665, D-78457 Constance, Germany; Univ Konstanz, Fachbereich Biol, Math Nat Wissensch Sekt, D-78457 Constance, Germany
Identifiers:ISI:000173442200030 [ID No:1]
ISSN:0907-4449 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.