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          Institute: MPI für molekulare Biomedizin     Collection: Publikationen molekulare Biomedizin     Display Documents



ID: 357252.0, MPI für molekulare Biomedizin / Publikationen molekulare Biomedizin
Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation
Authors:Varga, G.; Balkow, S.; Wild, M. K.; Stadtbaeumer, A.; Krummen, M.; Rothoeft, T.; Higuchi, T.; Beissert, S.; Wethmar, K.; Scharffetter-Kochanek, K.; Vestweber, D.; Grabbe, S.
Date of Publication (YYYY-MM-DD):2007
Title of Journal:Blood
Volume:109
Issue / Number:2
Start Page:661
End Page:669
Audience:Not Specified
Abstract / Description:The beta2 integrins are important for transendothelial migration of leukocytes as well as for T-cell activation during antigen presentation. Despite abundant expression of beta2 integrins on antigen-presenting cells (APCs), their functional relevance for antigen presentation is completely unclear. We show here that dendritic cells (DCs) from CD18-deficient mice, which lack all functional beta2 integrins, have no defect in antigen presentation. Moreover, DCs from normal mice express inactive beta2 integrins that do not become activated on contact with T cells, at least in vitro. Pharmacologic activation of beta2 integrins on DCs results in a significant reduction of their T cell-activating capacity. This effect is mediated by Mac-1 (CD11b/CD18) on DCs because it could be reversed via blocking antibodies against CD18 and CD11b. Furthermore, the antigen-presenting capacity of macrophages, which express constitutively active beta2 integrins, is significantly enhanced on Mac-1 blockade. We therefore conclude that active CD11b/CD18 (Mac-1) on APCs directly inhibits T-cell activation.
Free Keywords:Animals Antigen Presentation/immunology Antigens, CD11b/*physiology Antigens, CD18/genetics/metabolism/*physiology Cells, Cultured Dendritic Cells/*immunology Immunophenotyping Lymphocyte Activation/*immunology Macrophage-1 Antigen/*physiology Macrophages/immunology Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout T-Lymphocytes/*immunology
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Biomedizin
Identifiers:URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...
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