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          Institute: MPI für Evolutionsbiologie     Collection: Ecophysiology     Display Documents



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ID: 367584.0, MPI für Evolutionsbiologie / Ecophysiology
Habitat partitioning of denitrifying bacterial communities carrying nirS or nirK genes in the stratified water column of Lake Kinneret, Israel
Authors:Junier, Pilar; Kim, Ok-Sun; Witzel, Karl-Paul; Imhoff, Johannes F.; Hadas, Ora
Language:English
Date of Publication (YYYY-MM-DD):2008-05-19
Title of Journal:Aquatic Microbial Ecology
Journal Abbrev.:Aquat Microb Ecol
Volume:51
Issue / Number:2
Start Page:129
End Page:140
Review Status:not specified
Audience:Not Specified
Abstract / Description:The community composition of denitrifying bacteria was studied in the stratified water column of Lake Kinneret. The nitrite reductase genes nirS and nirK were amplified by PCR from water samples taken at 1, 14, 19 and 22 m depth, which represent the epi-, meta- and hypolimnion of the lake. The PCR products were analyzed with terminal restriction fragment length polymorphism (T-RFLP) and clone libraries. The highest diversity of nirS denitrifying communities was observed at 1 m depth. According to the T-RFLP profiles and clone libraries of nirS products, 2 groups of denitrifiers were common to and dominant in all depths. Deduced protein sequences from one of these groups displayed low identity (77 %) with other nirS sequences reported in GenBank. Denitrifying bacterial communities with nirK were most diverse at 22 m and showed highest similarity to those at 19 m depth. Sequences unrelated to nirK dominated the clone libraries from 1 m depth, suggesting that denitrifying bacteria with copper-containing nitrite reductase were less frequent at this depth. The results suggest that microorganisms with nirK and those with nirS respond differently to the environmental conditions in the stratified water column of Lake Kinneret.
Free Keywords:nirS; nirK; T-RFLP; Lake Kinneret
External Publication Status:published
Document Type:Article
Affiliations:MPI für Evolutionsbiologie/Abt. Ökophysiologie
External Affiliations:Ecole Polytech Fed Lausanne, CH-1015 Lausanne, Switzerland;
Univ Kiel, Leibniz Inst Marine Sci, D-24105 Kiel, Germany;
Kinneret Limnol Lab, IL-14950 Migdal, Israel
Identifiers:ISSN:0948-3055 [ID-No:1]
DOI:10.3354/ame01186 [ID-No:2]
LOCALID:2617/S 38794 [Listen-Nummer/S-Nummer]
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