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          Institute: MPI für Biochemie     Collection: Independent Research Groups     Display Documents



ID: 39023.0, MPI für Biochemie / Independent Research Groups
Characterization of the macrocyclic carbon suboxide factors as potent Na,K-ATPase and SR Ca-ATPase inhibitors
Authors:Kerek, F.; Stimac, R.; Apell, H. J.; Freudenmann, F.; Moroder, L.
Language:English
Date of Publication (YYYY-MM-DD):2002-12-23
Title of Journal:Biochimica et Biophysica Acta-Biomembranes
Journal Abbrev.:Biochim. Biophys. Acta-Biomembr.
Volume:1567
Issue / Number:1-2
Start Page:213
End Page:220
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Recently discovered macrocyclic carbon suboxide (MCS) factors with the general formula (C3O2)(n) were found to strongly inhibit rabbit and rat Na,K-ATPase as well as SR Ca-ATPase. Highly active MCS factors were obtained by a base/acid treatment of their lipophilic precursor isolated from plants. In the ESI-MS spectra, the dominant molar mass ion of 431 Da corresponds to a 1:1 complex of the carbon suboxide hexamer (n = 6; M-r = 408 Da) with a Na+ ion. Additional mass ions identified in positive and negative ion mode were assigned as complexes of the MCS hexamer (n = 6) and octamer (n = 8) with Na+ or with TFA(-) in various ratios. The dominant mass ion values of these active MCS factors from plants are also found in mass spectra of previously described endogenous digitalis- like factors (EDLF) from animals. This would suggest that ubiquitously distributed MCS factors may function as putative endogenous regulatory substances of Na,K-ATPase and possibly of other ATPases. With the symmetric display of several equivalent carbonyl or hydroxy groups, the structure of MCS factors is particularly suited for interactions with proteins and other bio-molecules. This could explain the high biological activity and the unusual properties of the MCS factors. (C) 2002 Elsevier Science B.V. All rights reserved.
Free Keywords:macrocyclic carbon suboxide; Na;K-ATPase; Ca-ATPase; endogenous regulatory factor; enzymatic activity
Comment of the Author/Creator:Date: 2002, DEC 23
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Independent Research Groups/Bioorganic Chemistry (L. Moroder)
External Affiliations:Univ Konstanz, Dept Biol, D-78457 Constance, Germany
Identifiers:ISI:000180323100025 [ID No:1]
ISSN:0005-2736 [ID No:2]
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