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          Institute: MPI für Biochemie     Collection: Cell Biology (E. A. Nigg)     Display Documents

ID: 39326.0, MPI für Biochemie / Cell Biology (E. A. Nigg)
The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo
Authors:Grüneberg, U.; Glotzer, M.; Gartner, A.; Nigg, E. A.
Date of Publication (YYYY-MM-DD):2002-09-02
Title of Journal:Journal of Cell Biology
Journal Abbrev.:J. Cell Biol.
Issue / Number:5
Start Page:901
End Page:914
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In all eukaryotic organisms, the physical separation of two nascent cells must be coordinated with chromosome segregation and mitotic exit. In Saccharomyces cerevisiae and Schizosaccharomyces pombe this coordination depends on a number of genes that cooperate in intricate regulatory pathways termed mitotic exit network and septum initiation network, respectively. Here we have explored the function of potentially homologous genes in a metazoan organism, Caenorhabditis elegans, using RNA-mediated interference. Of all the genes tested, only depletion of CeCDC-14, the C. elegans homologue of the budding yeast dual-specificity phosphatase Cdc14p (Clp1/Flp1p in fission yeast), caused embryonic lethality. We show that CeCDC-14 is required for cytokinesis but may be dispensable for progression of the early embryonic cell cycles. In response to depletion of CeCDC-14, embryos fail to establish a central spindle, and several proteins normally found at this structure are mislocalized. CeCDC-14 itself localizes to the central spindle in anaphase and to the midbody in telophase. It colocalizes with the mitotic kinesin ZEN-4, and the two proteins depend on each other for correct localization. These findings identify the CDC14 phosphatase as an important regulator of central spindle formation and cytokinesis in a metazoan organism.
Free Keywords:CDC14; cytokinesis; central spindle; ZEN-4; AIR-2
Comment of the Author/Creator:Date: 2002, SEP 2
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Cell Biology (E.A. Nigg)/DNA Damage Response and Cell Cycle Regulation in C. elegans (A. Gartner)
External Affiliations:Res Inst Mol Pathol, A-1030 Vienna, Austria
Identifiers:ISI:000177843000008 [ID No:1]
ISSN:0021-9525 [ID No:2]
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