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          Institute: MPI für Biochemie     Collection: Molecular Medicine (R. Fässler)     Display Documents

ID: 39346.0, MPI für Biochemie / Molecular Medicine (R. Fässler)
A crucial role of beta 1 integrins for keratinocyte migration in vitro and during cutaneous wound repair
Authors:Grose, R.; Hutter, C.; Bloch, W.; Thorey, I.; Watt, F. M.; Fässler, R.; Brakebusch, C.; Werner, S.
Date of Publication (YYYY-MM-DD):2002-05
Title of Journal:Development
Journal Abbrev.:Development
Issue / Number:9
Start Page:2303
End Page:2315
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Integrins are ubiquitous transmembrane receptors that play crucial roles in cell-cell and cell-matrix interactions. In this study, we have determined the effects of the loss of beta1 integrins in keratinocytes in vitro and during cutaneous wound repair. Flow cytometry of cultured beta1-deficient keratinocytes confirmed the absence of beta1 integrins and showed downregulation of alpha6beta4 but not of alphanu integrins. beta1-null keratinocytes were characterised by poor adhesion to various substrates, by a reduced proliferation rate and by a strongly impaired migratory capacity. In vivo, the loss of 01 integrins in keratinocytes caused a severe defect in wound healing. beta1-null keratinocytes showed impaired migration and were more densely packed in the hyperproliferative epithelium. Surprisingly, their proliferation rate was not reduced in early wounds and even increased in late wounds. The failure in re-epithelialisation resulted in a prolonged inflammatory response, leading to dramatic alterations in the expression of important wound- regulated genes. Ultimately, beta1-deficient epidermis did cover the wound bed, but the epithelial architecture was abnormal. These findings demonstrate a crucial role of beta1 integrins in keratinocyte migration and wound re- epithelialisation.
Free Keywords:integrin; migration; epidermis; mouse; wound
Comment of the Author/Creator:Date: 2002, MAY
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Molecular Medicine (R. Fässler)
MPI für Biochemie/Independent Junior Research Groups/Regulation of Cytoskeletal Organisation (C. Brakebusch, Department of Molecular Medicine, Heisenberg)
External Affiliations:ETH Zurich, Dept Biol, Inst Cell Biol, CH-8093 Zurich,; Switzerland; ETH Zurich, Dept Biol, Inst Cell Biol, CH-8093 Zurich, Switzerland; Canc Res UK London Res Inst, Keratinocyte Lab, London WC2A 3PX, England; Univ Cologne, Inst Anat, D-50931 Cologne, Germany; Lund Univ, Dept Expt Pathol, S-22185 Lund, Sweden
Identifiers:ISI:000175694400022 [ID No:1]
ISSN:0950-1991 [ID No:2]
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