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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



ID: 402889.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Reduced V-ATPase activity in the trans-Golgi network causes oxylipin-dependent hypocotyl growth Inhibition in Arabidopsis
Authors:Brux, A.; Liu, T. Y.; Krebs, M.; Stierhof, Y. D.; Lohmann, J. U.; Miersch, O.; Wasternack, C.; Schumacher, K.
Date of Publication (YYYY-MM-DD):2008-04
Journal Abbrev.:Plant Cell
Volume:20
Issue / Number:4
Start Page:1088
End Page:1100
Audience:Not Specified
Abstract / Description:Regulated cell expansion allows plants to adapt their morphogenesis to prevailing environmental conditions. Cell expansion is driven by turgor pressure created by osmotic water uptake and is restricted by the extensibility of the cell wall, which in turn is regulated by the synthesis, incorporation, and cross-linking of new cell wall components. The vacuolar H(+)-ATPase (V-ATPase) could provide a way to coordinately regulate turgor pressure and cell wall synthesis, as it energizes the secondary active transport of solutes across the tonoplast and also has an important function in the trans-Golgi network (TGN), which affects synthesis and trafficking of cell wall components. We have previously shown that det3, a mutant with reduced V-ATPase activity, has a severe defect in cell expansion. However, it was not clear if this is caused by a defect in turgor pressure or in cell wall synthesis. Here, we show that inhibition of the tonoplast-localized V-ATPase subunit isoform VHA-a3 does not impair cell expansion. By contrast, inhibition of the TGN-localized isoform VHA-a1 is sufficient to restrict cell expansion. Furthermore, we provide evidence that the reduced hypocotyl cell expansion in det3 is conditional and due to active, hormone-mediated growth inhibition caused by a cell wall defect.
Free Keywords:Arabidopsis/enzymology/genetics/*growth & development; Cellulose/biosynthesis; Ethylenes/metabolism; Gene Expression Profiling; Golgi Apparatus/*enzymology; Hypocotyl/enzymology/*growth & development; Microscopy, Electron, Transmission; Oxylipins/*metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; Vacuolar Proton-Translocating ATPases/*metabolism
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)/
External Affiliations:%G eng
Identifiers:ISSN:1040-4651 (Print) %R tpc.108.058362 [pii] 10.1105/tpc.108.058362
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=...
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