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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents

ID: 402899.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Dual roles of the nuclear cap-binding complex and SERRATE in pre-mRNA splicing and microRNA processing in Arabidopsis thaliana
Authors:Laubinger, S.; Sachsenberg, T.; Zeller, G.; Busch, W.; Lohmann, J. U.; Ratsch, G.; Weigel, D.
Date of Publication (YYYY-MM-DD):2008-06-24
Journal Abbrev.:Proc Natl Acad Sci U S A
Issue / Number:25
Start Page:8795
End Page:8800
Audience:Not Specified
Abstract / Description:The processing of Arabidopsis thaliana microRNAs (miRNAs) from longer primary transcripts (pri-miRNAs) requires the activity of several proteins, including DICER-LIKE1 (DCL1), the double-stranded RNA-binding protein HYPONASTIC LEAVES1 (HYL1), and the zinc finger protein SERRATE (SE). It has been noted before that the morphological appearance of weak se mutants is reminiscent of plants with mutations in ABH1/CBP80 and CBP20, which encode the two subunits of the nuclear cap-binding complex. We report that, like SE, the cap-binding complex is necessary for proper processing of pri-miRNAs. Inactivation of either ABH1/CBP80 or CBP20 results in decreased levels of mature miRNAs accompanied by apparent stabilization of pri-miRNAs. Whole-genome tiling array analyses reveal that se, abh1/cbp80, and cbp20 mutants also share similar splicing defects, leading to the accumulation of many partially spliced transcripts. This is unlikely to be an indirect consequence of improper miRNA processing or other mRNA turnover pathways, because introns retained in se, abh1/cbp80, and cbp20 mutants are not affected by mutations in other genes required for miRNA processing or for nonsense-mediated mRNA decay. Taken together, our results uncover dual roles in splicing and miRNA processing that distinguish SE and the cap-binding complex from specialized miRNA processing factors such as DCL1 and HYL1.
Free Keywords:Arabidopsis/*genetics/metabolism; Calcium-Binding Proteins/*metabolism; Intercellular Signaling Peptides and Proteins/*metabolism; Membrane Proteins/*metabolism; MicroRNAs/*metabolism; Nuclear Cap-Binding Protein Complex/*metabolism; RNA Precursors/*metabolism; RNA Processing, Post-Transcriptional; *RNA Splicing; RNA, Messenger/*metabolism
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)/
External Affiliations:%G eng
Identifiers:ISSN:1091-6490 (Electronic) %R 0802493105 [pii] 10.1073/pnas.0802493105
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