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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents

ID: 403308.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon
Authors:Hain, Torsten; Hossain, Hamid; Chatterjee, Som S; Machata, Silke; Volk, Ute; Wagner, Sandra; Brors, Benedikt; Haas, Stefan; Kuenne, Carsten T; Billion, Andre; Otten, Sonja; Pane-Farre, Jan; Engelmann, Susanne; Chakraborty, Trinad
Date of Publication (YYYY-MM-DD):2008-01-28
Title of Journal:BMC Microbiology
Issue / Number:20
Copyright:© 2008 Hain et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Background
The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, σB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine σB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify σB-dependent genes at different growth phases.
We detected 105 σB-positively regulated genes and 111 genes which appeared to be under negative control of σB and validated 36 σB-positively regulated genes in vivo using a reporter gene fusion system.
Genes comprising the σB regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. Ten of the σB-positively regulated genes of L. monocytogenes are absent in L. innocua. A total of 75 σB-positively regulated listerial genes had homologs in B. subtilis, but only 33 have been previously described as being σB-regulated in B. subtilis even though both species share a highly conserved σB-dependent consensus sequence. A low overlap of genes may reflects adaptation of these bacteria to their respective environmental conditions.
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Institute for Medical Microbiology, Justus-Liebig-University, Frankfurter Strasse 107, D-35392 Giessen, Germany;
2.Div. Intell. Bioinformatics Systems, DKFZ, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany;
3.Ernst-Moritz-Arndt-University, Institute for Microbiology, Jahnstraße 15, D-17487 Greifswald, Germany.
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