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          Institute: MPI für Infektionsbiologie     Collection: Molecular Immunology     Display Documents

ID: 403586.0, MPI für Infektionsbiologie / Molecular Immunology
Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
Authors:Tiller, Thomas; Meffre, Eric; Yurasov, Sergey; Tsuiji, Makoto; Nussenzweig, Michel C.; Wardemann, Hedda
Date of Publication (YYYY-MM-DD):2008-01-01
Title of Journal:Journal of Immunological Methods
Journal Abbrev.:J. Immunol. Methods
Issue / Number:1-2
Start Page:112
End Page:124
Copyright:Copyright © 2007 Elsevier B.V. All rights reserve
Review Status:not specified
Audience:Experts Only
Free Keywords:antibody; single cell PCR; self-reactivity; polyreactivity
Comment of the Author/Creator:Acknowledgements
We thank all members of the Nussenzweig and Wardemann laboratory for discussion and S. Boscardin for helpful comments on the manuscript. We are indebted to Patrick Wilson and Anne Schaefer for their help and Aaron B. Kantor for advice on cDNA synthesis from single cells. This work was supported by grants from the National Institutes of Health (to M.C. Nussenzweig, E. Meffre) and the Dana Foundation Human Immunology Program (to E. Meffre, H. Wardemann). S. Yurasov is a Charles H. Revson Fellow in Biomedical Research and is supported by the Charles A. Dana Foundation. M.C. Nussenzweig is an investigator of the Howard Hughes Medical Institute.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:N. N.
Affiliations:MPI für Infektionsbiologie/Molecular Immunology
External Affiliations:Hosp Special Surg, Biochem & Mol Biol Lab, New York, NY 10021 USA.; Rockefeller Univ, Lab Mol Immnunol, New York, NY 10021 USA.; Mem Sloan Kettering Canc Ctr, Dept Pediat, New York, NY 10021 USA.; Tokyo Med & Dent Univ, Med Res Inst, Dept Immunol, Tokyo, Japan.; Howard Hughes Med Inst, New York, NY 10021 USA.
Identifiers:ISI:000253124100011 [ID No:1]
ISSN:0022-1759 [ID No:2]
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