Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents



  history
ID: 405603.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase
Authors:Tegha-Dunghu, Justus; Neumann, Beate; Krause, Roland; Erfle, Holger; Walter, Thomas; Held, Michael; Rogers, Phill; Hupfeld, Kerstin; Ruppert, Thomas; Ellenberg, Jan; Gruss, Oliver J.
Language:English
Date of Publication (YYYY-MM-DD):2008-04-29
Title of Journal:Journal of Cell Science
Volume:121
Start Page:1718
End Page:1726
Copyright:© The Company of Biologists Ltd 2008
Review Status:not specified
Audience:Experts Only
Abstract / Description:Assembly of the mitotic spindle requires a global change in the activity and constitution of the microtubule-binding-protein array at mitotic onset. An important subset of mitotic microtubule-binding proteins localises to the nucleus in interphase and essentially contributes to spindle formation and function after nuclear envelope breakdown. Here, we used a proteomic approach to selectively identify proteins of this category and revealed 50 poorly characterised human gene products, among them the echinoderm microtubule-associated-protein-like gene product, EML3. Indirect immunofluorescence showed that EML3 colocalises with spindle microtubules throughout all mitotic stages. In interphase, EML3 colocalised with cytoplasmic microtubules and accumulated in interphase nuclei. Using YFP-fusion constructs of EML3, we located a nuclear localisation signal and confirmed the microtubule-binding domain of EML3. Functional analysis of EML3 using time-lapse fluorescence microscopy and detailed end-point analysis of phenotypes after siRNA knockdown demonstrates an important role for EML3 in correct metaphase chromosome alignment. Our proteomic identification screen combined with sensitive phenotypic analysis therefore provides a reliable platform for the identification and characterisation of proteins important for correct cell division.
Free Keywords:Spindle proteins, Nuclear Proteins, EML3
Comment of the Author/Creator:Author for correspondence:
e-mail: o.gruss@zmbh.uni-heidelberg.de
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany;
2.MitoCheck Project Group, European Molecular Biology Laboratory, Meyerhofstr.1, 69117 Heidelberg, Germany.
Identifiers:URL:http://jcs.biologists.org/cgi/content/abstract/121...
DOI:10.1242/jcs.019174
ISSN:0021-9533
Full Text:
Sorry, no privileges
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.