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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 411029.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex
Authors:Lange, Martin; Kaynak, Bogac; Forster, Ulrike B.; Tönjes, Martje; Fischer, Jenny J.; Grimm, Christina; Schlesinger, Jenny; Just, Steffen; Dunkel, Ilona; Krueger, Tammo; Mebus, Siegrun; Lehrach, Hans; Lurz, Rudi; Gobom, Johan; Rottbauer, Wolfgang; Abdelilah-Seyfried, Salim; Sperling, Silke
Language:English
Date of Publication (YYYY-MM-DD):2008-09-01
Title of Journal:Genes and Development
Journal Abbrev.:Genes Dev
Volume:22
Issue / Number:17
Start Page:2370
End Page:2384
Copyright:© 2008 by Cold Spring Harbor Laboratory Press
Review Status:not specified
Audience:Experts Only
Abstract / Description:Chromatin remodeling and histone modifications facilitate access of transcription factors to DNA by promoting the unwinding and destabilization of histone–DNA interactions. We present DPF3, a new epigenetic key factor for heart and muscle development characterized by a double PHD finger. DPF3 is associated with the BAF chromatin remodeling complex and binds methylated and acetylated lysine residues of histone 3 and 4. Thus, DPF3 may represent the first plant homeodomains that bind acetylated lysines, a feature previously only shown for the bromodomain. During development Dpf3 is expressed in the heart and somites of mouse, chicken, and zebrafish. Morpholino knockdown of dpf3 in zebrafish leads to incomplete cardiac looping and severely reduced ventricular contractility, with disassembled muscular fibers caused by transcriptional deregulation of structural and regulatory proteins. Promoter analysis identified Dpf3 as a novel downstream target of Mef2a. Taken together, DPF3 adds a further layer of complexity to the BAF complex by representing a tissue-specific anchor between histone acetylations as well as methylations and chromatin remodeling. Furthermore, this shows that plant homeodomain proteins play a yet unexplored role in recruiting chromatin remodeling complexes to acetylated histones.
Free Keywords:Heart and skeletal muscle development and function, PHD finger, BAF chromatin remodeling complex, SMARCD3–BAF60 acetylated and methylated histones, Mef2
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Cell Polarity and Epithelial Development, Max Delbrück Center, Berlin 13125 Germany
Molecular Cardiology, Ruprecht-Karls-Universität Heidelberg, Heidelberg 69120, Germany
Department Pediatric Cardiology, German Heart Center Berlin, Berlin 13353, Germany
Identifiers:ISSN:0890-9369
DOI:10.1101/gad.471408
URL:http://genesdev.cshlp.org/content/22/17/2370.full....
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