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          Institute: MPI für Biochemie     Collection: Cell Biology (E. A. Nigg)     Display Documents

ID: 41118.0, MPI für Biochemie / Cell Biology (E. A. Nigg)
Human TPX2 is required for targeting Aurora-A kinase to the spindle
Authors:Kufer, T. A.; Sillje, H. H. W.; Körner, R.; Gruss, O. J.; Meraldi, P.; Nigg, E. A.
Date of Publication (YYYY-MM-DD):2002-08-19
Title of Journal:Journal of Cell Biology
Journal Abbrev.:J. Cell Biol.
Issue / Number:4
Start Page:617
End Page:623
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Aurora-A is a serine-threonine kinase implicated in the assembly and maintenance of the mitotic spindle. Here we show that human Aurora-A binds to TPX2, a prominent component of the spindle apparatus. TPX2 was identified by mass spectrometry as a major protein coimmunoprecipitating specifically with Aurora- A from mitotic HeLa cell extracts. Conversely, Aurora-A could be detected in TPX2 immunoprecipitates. This indicates that subpopulations of these two proteins undergo complex formation in vivo. Binding studies demonstrated that the NH2 terminus of TPX2 can directly interact with the COOH-terminal catalytic domain of Aurora-A. Although kinase activity was not required for this interaction, TPX2 was readily phosphorylated by Aurora-A. Upon siRNA-mediated elimination of TPX2 from cells, the association of Aurora-A with the spindle microtubules was abolished, although its association with spindle poles was unaffected. Conversely, depletion of Aurora-A by siRNA had no detectable influence on the localization of TPX2. We propose that human TPX2 is required for targeting Aurora-A kinase to the spindle apparatus. In turn, Aurora-A might regulate the function of TPX2 during spindle assembly.
Free Keywords:spindle; mitosis; Aurora-A; TPX2; siRNA
Comment of the Author/Creator:Date: 2002, AUG 19
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Cell Biology (E.A. Nigg)/Mitotic Spindle (H. Silljé)
MPI für Biochemie/Cell Biology (E.A. Nigg)/Mass Spectrometry (R. Körner)
External Affiliations:European Mol Biol Lab, D-69117 Heidelberg, Germany
Identifiers:ISI:000177649900003 [ID No:1]
ISSN:0021-9525 [ID No:2]
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