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          Institute: MPI für molekulare Genetik     Collection: Microscopy     Display Documents

ID: 412097.0, MPI für molekulare Genetik / Microscopy
Spongiibacter marinus gen. nov., sp. nov., a halophilic marine bacterium isolated from the boreal sponge Haliclona sp. 1
Authors:Graeber, Ingeborg; Kaesler, Ines; Borchert, Martin S.; Dieckmann, Ralf; Pape, Thomas; Lurz, Rudi; Nielsen, Preben; von Döhren, Hans; Michaelis, Walter; Szewzyk, Ulrich
Date of Publication (YYYY-MM-DD):2008-03
Title of Journal:International Journal of Sytematic and Evolutionary Micro
Journal Abbrev.:Int J Syst Evol Microbiol
Issue / Number:Pt 3
Start Page:585
End Page:590
Copyright:© 2008 by the International Union of Microbiological Societies.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Strain HAL40bT was isolated from the marine sponge Haliclona sp. 1 collected at the Sula Ridge off the Norwegian coast and characterized by physiological, biochemical and phylogenetic analyses. The isolate was a small rod with a polar flagellum. It was aerobic, Gram-negative and oxidase- and catalase-positive. Optimal growth was observed at 20–30 °C, pH 7–9 and in 3 % NaCl. Substrate utilization tests were positive for arabinose, Tween 40 and Tween 80. Enzyme tests were positive for alkaline phosphatase, esterase lipase (C8), leucine arylamidase, acid phosphatase, naphthol-AS-BI-phosphohydrolase and N-acetyl-β-glucosaminidase. The predominant cellular fatty acid was C17 : 1{omega}8, followed by C17 : 0 and C18 : 1{omega}7. Analysis by matrix-assisted laser desorption/ionization time-of-flight MS was used to characterize the strain, producing a characteristic low-molecular-mass protein pattern that could be used as a fingerprint for identification of members of this species. The DNA G+C content was 69.1 mol%. Phylogenetic analysis supported by 16S rRNA gene sequence comparison classified the strain as a member of the class Gammaproteobacteria. Strain HAL40bT was only distantly related to other marine bacteria including Neptunomonas naphthovorans and Marinobacter daepoensis (type strain sequence similarity >90 %). Based on its phenotypic, physiological and phylogenetic characteristics, it is proposed that the strain should be placed into a new genus as a representative of a novel species, Spongiibacter marinus gen. nov., sp. nov.; the type strain of Spongiibacter marinus is HAL40bT (=DSM 17750T =CCUG 54896T).
Comment of the Author/Creator:Correspondence
Ingeborg Graeber
External Publication Status:published
Document Type:Article
Communicated by:Rudi Lurz
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Berlin University of Technology, Environmental Microbiology Group, Franklinstrasse 29, Sekr. FR 1-2, D-10587 Berlin, Germany;
2.Hamburg University of Technology, Technical Microbiology, Kasernenstrasse 12, D-21073 Hamburg, Germany;
3.Anagnostec GmbH, Am Mühlenberg 11, D-14476 Potsdam-Golm, Germany;
4.University of Hamburg, Institute of Biogeochemistry and Marine Chemistry, Bundesstrasse 55, D-20146 Hamburg, Germany
5.Novozymes A/S, Bacterial screening, Krogshøjvej 36, DK-2880 Bagsværd, Denmark;
6.Berlin University of Technology, Biochemistry and Molecular Biology Group, Franklinstrasse 29, Sekr. OE2, D-10587 Berlin, Germany.
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