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          Institute: MPI für molekulare Genetik     Collection: Ribosome Research Groups     Display Documents



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ID: 412540.0, MPI für molekulare Genetik / Ribosome Research Groups
In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs
Authors:Stelz, Ulrich; Nierhaus, Knud
Language:English
Place of Publication:Berlin [et al]
Publisher:Springer
Date of Publication (YYYY-MM-DD):2008-10-01
Title of Book:RNA-Protein Interaction Protocols
Start Page:247
End Page:255
Full Name of Book-Editor(s):Ren-Jang Lin
Title of Series:Methods in Molecular Biology
Volume:488
Copyright:Springer
Review Status:not specified
Audience:Experts Only
Abstract / Description:In vitro selection experiments have various goals depending on the composition of the initial pool and the selection method applied. We developed an in vitro selection variant that is useful for the identification of minimal RNA binding sites for proteins within large RNAs. A pool of randomly fragmented RNA is constructed from a large RNA, which is the natural binding partner for a protein. Such a pool contains all the potential binding sites and is therefore used as starting material for affinity selection. A successful in vitro selection with the purified protein will identify the protein's natural RNA target site. The method has been developed for ribosomal systems and is a general approach providing a basis for the functional and structural characterization of large ribonucleoprotein particles.
Free Keywords:In vitro selection - ribosome - RNA binding site - RNA-protein interaction - SELEX - SERF
External Publication Status:published
Document Type:InBook
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
Identifiers:URL:http://www.springerprotocols.com/Abstract/doi/10.1...
DOI:10.1007/978-1-60327-475-3_17
ISBN:978-1-58829-419-7
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