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          Institute: MPI für molekulare Genetik     Collection: Ribosome Research Groups     Display Documents



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ID: 412548.0, MPI für molekulare Genetik / Ribosome Research Groups
Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome
Authors:Bishop, A. Özlem Tastan; Stelzl, Ulrich; Pech, Markus; Nierhaus, Knud H.
Language:English
Place of Publication:Berlin [et al]
Publisher:Springer
Date of Publication (YYYY-MM-DD):2008-10-01
Title of Book:RNA-Protein Interaction Protocols
Start Page:129
End Page:151
Full Name of Book-Editor(s):Ren-Jang Lin
Title of Series:Methos of molecular biology
Volume:488
Copyright:Springer
Review Status:not specified
Audience:Experts Only
Abstract / Description:Analogs of naturally occurring substances obtained by chemical modifications are powerful tools to study intra- and intermolecular interactions. We have used the phosphorothioate technique to analyze RNA-protein interactions, here the interactions of transfer RNAs (tRNAs) with the three ribosomal binding sites. We describe preparation and purification of thioated tRNAs, formation of functional complexes of programmed ribosomes with tRNAs, and the evaluation of the observed phosphorothioate footprints on the tRNAs.
Free Keywords:Phosphorothioate technique - ribosomal tRNA contact sites - RNA-protein interactions
External Publication Status:published
Document Type:InBook
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Bioinformatics and Computational Biology Unit, Department of Biochemistry, University of Pretoria, Pretoria, South Africa.
Identifiers:URL:http://www.springerprotocols.com/BookToc/doi/10.10...
DOI:10.1007/978-1-60327-475-3_9
ISBN:978-1-58829-419-7
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