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          Institute: MPI für Neurobiologie     Collection: Molecular Neurobiology     Display Documents

ID: 412743.0, MPI für Neurobiologie / Molecular Neurobiology
Absence of functional peroxisomes from mouse CNS causes dysmyelination and axon degeneration
Authors:Hulshagen, L.; Krysko, O.; Bottelbergs, A.; Huyghe, S.; Klein, R.; Van Veldhoven, P. P.; De Deyn, P. P.; D'Hooge, R.; Hartmann, D.; Baes, M.
Date of Publication (YYYY-MM-DD):2008-04-09
Title of Journal:Journal of Neuroscience
Journal Abbrev.:J. Neurosci.
Issue / Number:15
Start Page:4015
End Page:4027
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Peroxisomal metabolism is essential for normal brain development both in men and in mice. Using conditional knock-out mice, we recently showed that peroxisome deficiency in liver has a severe and persistent impact on the formation of cortex and cerebellum, whereas absence of functional peroxisomes from the CNS only causes developmental delays without obvious alteration of brain architecture. We now report that a substantial fraction of the latter Nes-Pex5 knock-out mice survive into adulthood but develop progressive motoric and coordination problems, impaired exploration, and a deficit in cognition and die before the age of 6 months. Histopathologically, both the white and gray matter of the CNS displayed a region-specific accumulation of neutral lipids, astrogliosis and microgliosis, upregulation of catalase, and scattered cell death. Nes-Pex5 knock-out mice featured a dramatic reduction of myelin staining in corpus callosum, whereas cerebellum and other white matter tracts were less affected or unchanged. This was accompanied by a depletion of alkenylphospholipids in myelin and differentially reduced immunoreactivity of myelin proteins. EM analysis revealed that myelin wrappings around axons did still form, but they showed a reduction in thickness relative to axon diameters. Remarkably, multifocal axonal damage occurred in the corpus callosum. Thereby, debris accumulated between axolemma and inner myelin surface and axons collapsed, although myelin sheaths remained present. These anomalies of myelinated axons were already present in juvenile mice but aggravated in adulthood. Together, loss of CNS peroxisomal metabolism both affects myelin sheaths and axonal integrity possibly via independent pathways.
Free Keywords:peroxisome; myelin; Zellweger syndrome; plasmalogen; fatty acid; knock-out mice; astrocyte; axon; microglia
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Affiliations:MPI für Neurobiologie/Molecular Neurobiology (Klein)
External Affiliations:[Hulshagen, Leen; Krysko, Olga; Bottelbergs, Astrid; Huyghe, Steven; Baes, Myriam] Katholieke Univ Leuven, Dept Pharmaceut Sci, Lab Cell Metab, B-3000 Louvain, Belgium.; [Van Veldhoven, Paul P.] Katholieke Univ Leuven, Dept Mol Cell Biol, Lab Lipid Biochem & Prot Interact, B-3000 Louvain, Belgium.; [Hartmann, Dieter] Katholieke Univ Leuven, Ctr Human Genet, B-3000 Louvain, Belgium.; [Hartmann, Dieter] Katholieke Univ Leuven VIB, Dept Mol & Dev Genet, B-3000 Louvain, Belgium.; [De Deyn, Peter P.; D'Hooge, Rudi] Univ Antwerp, Lab Neurochem & Behav, B-2610 Antwerp, Belgium.; [Hartmann, Dieter] Univ Bonn, Dept Anat, D-53115 Bonn, Germany.
Identifiers:ISI:000255012400021 [ID No:1]
ISSN:0270-6474 [ID No:2]
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