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          Institute: MPI für Biochemie     Collection: Emeriti Groups     Display Documents



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ID: 41346.0, MPI für Biochemie / Emeriti Groups
Endosome fusion and microtubule-based dynamics in the early endocytic pathway of Dictyostelium
Authors:Clarke, M.; Köhler, J.; Heuser, J.; Gerisch, G.
Language:English
Date of Publication (YYYY-MM-DD):2002-11
Title of Journal:Traffic
Journal Abbrev.:Traffic
Volume:3
Issue / Number:11
Start Page:791
End Page:800
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Dictyostelium amoebae, like mammalian macrophages, take up fluid by macropinocytosis. The present study used fluorescent fluid-phase markers and GFP-labeled microtubules to visualize the uptake, dynamics, and fusion of early endosomes in Dictyostelium. Consecutive labeling with two fluorescent fluid- phase markers demonstrated that within the first few minutes after uptake, new macropinosomes underwent fusion with pre- existing endosomes. The fusing endosomes, which represent the mixing compartment, displayed extreme shape changes and rapid transport about the cell in association with microtubules. The great plasticity of endosomes at this stage of maturation was also evident by electron microscopy. The constant undulatory motion of microtubules was implemental in establishing contact with endosomes. Treatment of cells with agents that selectively disrupted either actin filaments or microtubules confirmed that endosome dynamics were microtubule based. Further maturation of endosomes led to loss of pleiomorphy in favor of a spherical shape, inability to fuse with new macropinosomes, and diminished motility.
Free Keywords:Dictyostelium; endosome; macropinocytosis; microtubule; vesicle traffic
Comment of the Author/Creator:Date: 2002, NOV
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Emeriti Groups/Cell Dynamics (G. Gerisch)
External Affiliations:Oklahoma Med Res Fdn, Program Mol & Cell Biol, 825 Ne 13Th St,; Oklahoma City, OK 73104 USA; Oklahoma Med Res Fdn, Program Mol & Cell Biol, Oklahoma City, OK 73104 USA; Washington Univ, Sch Med, St Louis, MO 63110 USA
Identifiers:ISI:000178617000004 [ID No:1]
ISSN:1398-9219 [ID No:2]
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