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          Institute: MPI für Biochemie     Collection: Cell Biology (E. A. Nigg)     Display Documents

ID: 41349.0, MPI für Biochemie / Cell Biology (E. A. Nigg)
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells
Authors:Gruss, O. J.; Wittmann, M.; Yokoyama, H.; Pepperkok, R.; Kufer, T.; Sillje, H.; Karsenti, E.; Mattaj, I. W.; Vernos, I.
Date of Publication (YYYY-MM-DD):2002-11
Title of Journal:Nature Cell Biology
Journal Abbrev.:Nat. Cell Biol.
Issue / Number:11
Start Page:871
End Page:879
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:In Xenopus laevis egg extracts, TPX2 is required for the Ran- GTP-dependent assembly of microtubules around chromosomes. Here we show that interfering with the function of the human homologue of TPX2 in HeLa cells causes defects in microtubule organization during mitosis. Suppressing the expression of human TPX2 by RNA interference leads to the formation of two microtubule asters that do not interact and do not form a spindle. Our results suggest that in vivo, even in the presence of duplicated centrosomes, spindle formation requires the function of TPX2 to generate a stable bipolar spindle with overlapping antiparallel microtubule arrays. This indicates that chromosome-induced microtubule production is a general requirement for the formation of functional spindles in animal cells.
Comment of the Author/Creator:Date: 2002, NOV
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Cell Biology (E.A. Nigg)/Mitotic Spindle (H. Silljé)
External Affiliations:European Mol Biol Lab, Meyerhofstr 1, D-69117 Heidelberg,; Germany; European Mol Biol Lab, D-69117 Heidelberg, Germany
Identifiers:ISI:000179137700016 [ID No:1]
ISSN:1465-7392 [ID No:2]
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