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          Institute: MPI für Biochemie     Collection: Molecular Medicine (R. Fässler)     Display Documents



ID: 41369.0, MPI für Biochemie / Molecular Medicine (R. Fässler)
Integrin-linked kinase regulates chondrocyte shape and proliferation
Authors:Grashoff, C.; Aszodi, A.; Sakai, T.; Hunziker, E. B.; Fässler, R.
Language:English
Date of Publication (YYYY-MM-DD):2003-04
Title of Journal:EMBO Reports
Journal Abbrev.:EMBO Rep.
Volume:4
Issue / Number:4
Start Page:432
End Page:438
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:The interaction of chondrocytes with the extracellular-matrix environment is mediated mainly by integrins. Ligated integrins are recruited to focal adhesions (FAs) together with scaffolding proteins and kinases, such as integrin-linked kinase (Ilk). Ilk binds the cytoplasmic domain of beta1-, beta2- and beta3-integrins and recruits adaptors and kinases, and is thought to stimulate downstream signalling events through phosphorylation of protein kinase B/Akt (Pkb/Akt) and glycogen synthase kinase 3-beta (GSK3-beta). Here, we show that mice with a chondrocyte-specific disruption of the gene encoding ilk develop chondrodysplasia, and die at birth due to respiratory distress. The chondrodysplasia was characterized by abnormal chondrocyte shape and decreased chondrocyte proliferation. In addition, Ilk-deficient chondrocytes showed adhesion defects, failed to spread and formed fewer FAs and actin stress fibres. Surprisingly, phosphorylation of Pkb/Akt and GSK3-beta is unaffected in Ilk-deficient chondrocytes. These findings suggest that Ilk regulates actin reorganization in chondrocytes and modulates chondrocyte growth independently of phosphorylation of Pkb/Akt and GSK3-beta.
Comment of the Author/Creator:Date: 2003, APR
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Molecular Medicine (R. Fässler)/Research Group Aszodi
External Affiliations:Univ Bern, ITI Res Inst Dent & Skeletal Biol, CH-3010 Bern, Switzerland
Identifiers:ISI:000182801300019 [ID No:1]
ISSN:1469-221X [ID No:2]
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