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          Institute: MPI für molekulare Genetik     Collection: Sequencing Group     Display Documents

ID: 413817.0, MPI für molekulare Genetik / Sequencing Group
Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L
Authors:Jurkowska, Renata Z.; Anspach, Nils; Urbanke, Claus; Jia, Da; Reinhardt, Richard; Nellen, Wolfgang; Cheng, Xiaodong; Jeltsch, Albert
Research Context:Gene regulation, Chromatin and Epigenetics
Date of Publication (YYYY-MM-DD):2008-10-22
Title of Journal:Nucleic Acids Research
Journal Abbrev.:Nucleic Acids Res
Issue / Number:21
Start Page:6656
End Page:6663
Copyright:© 2008 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review Status:not specified
Audience:Experts Only
Abstract / Description:The C-terminal domains of Dnmt3a and Dnmt3L form elongated heterotetramers (3L-3a-3a-3L). Analytical ultracentrifugation confirmed the Dnmt3a-C/3L-C complex exists as a 2:2 heterotetramer in solution. The 3a-3a interface is the DNA-binding site, while both interfaces are essential for AdoMet binding and catalytic activity. Hairpin bisulfite analysis shows correlated methylation of two CG sites in a distance of approximately 8-10 bp in the opposite DNA strands, which corresponds to the geometry of the two active sites in one Dnmt3a-C/3L-C tetramer. Correlated methylation was also observed for two CG sites at similar distances in the same DNA strand, which can be attributed to the binding of two tetramers next to each other. DNA-binding experiments show that Dnmt3a-C/3L-C complexes multimerize on the DNA. Scanning force microscopy demonstrates filament formation rather than binding of single tetramers and shows that protein-DNA filament formation leads to a 1.5-fold shortening of the DNA length.
Comment of the Author/Creator:To whom correspondence should be addressed: Tel: +49 421 200 3247; Fax: +49 421 200 3249; Email: a.jeltsch@jacobs-university.de
External Publication Status:published
Document Type:Article
Communicated by:Richard Reinhardt
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Biochemistry Lab, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen,Germany;
2.Abt. Genetik, CINSaT, Universität Kassel, Heinrich-Plett-Str. 40, 34132 Kassel,Germany;
3.Medizinische Hochschule, Abteilung Strukturanalyse OE 8830, Carl Neuberg Str. 1, 30625 Hannover, Germany;
4.Department of Biochemistry, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30033, USA.
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