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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2008     Display Documents



ID: 414323.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2008
A protein domain-based interactome network for C. elegans early embryogenesis
Authors:Boxem, Mike; Maliga, Zoltan; Klitgord, Niels; Li, Na; Lemmens, Irma; Mana, Miyeko; Lichtervelde, Lorenzo de; Mul, Joram D; Peut, Diederik van de; Devos, Maxime; Simonis, Nicolas; Yildirim, Muhammed A; Cokol, Murat; Kao, Huey-Ling; Smet, Anne-Sophie de; Wang, Haidong; Schlaitz, Anne-Lore; Hao, Tong; Milstein, Stuart; Fan, Changyu; Tipsword, Mike; Drew, Kevin; Galli, Matilde; Rhrissorrakrai, Kahn; Drechsel, David; Koller, Daphne; Roth, Frederick P; Iakoucheva, Lilia M; Dunker, A Keith; Bonneau, Richard; Gunsalus, Kristin C; Hill, David E; Piano, Fabio; Tavernier, Jan; Heuvel, Sander van den; Hyman, Anthony A; Vidal, Marc
Date of Publication (YYYY-MM-DD):2008
Title of Journal:Cell
Volume:134
Issue / Number:3
Start Page:534
End Page:545
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI f�r molekulare Zellbiologie und Genetik
Identifiers:LOCALID:985
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