Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2008     Display Documents



  history
ID: 414335.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2008
Quantum-dot-assisted characterization of microtubule rotations during cargo transport
Authors:Nitzsche, Bert; Ruhnow, Felix; Diez, Stefan
Date of Publication (YYYY-MM-DD):2008
Title of Journal:Nature Nanotechnology
Volume:3
Issue / Number:9
Start Page:552
End Page:556
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Owing to their wide spectrum of in vivo functions, motor proteins, such as kinesin-1, show great potential for application as nanomachines in engineered environments. When attached to a substrate surface, these motors are envisioned to shuttle cargo that is bound to reconstituted microtubules--one component of the cell cytoskeleton--from one location to another. One potentially serious problem for such applications is, however, the rotation of the microtubules around their longitudinal axis. Here we explore this issue by labelling the gliding microtubules with quantum dots to simultaneously follow their sinusoidal side-to-side and up-and-down motion in three dimensions with nanometre accuracy. Microtubule rotation, which originates from the kinesin moving along the individual protofilaments of the microtubule, was not impeded by the quantum dots. However, pick-up of large cargo inhibited the rotation but did not affect the velocity of microtubule gliding. Our data show that kinesin-driven microtubules make flexible, responsive and effective molecular shuttles for nanotransport applications.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:n.n.
Affiliations:MPI f�r molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1002
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.