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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2008     Display Documents



ID: 414398.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2008
Towards digital representation of Drosophila embryogenesis
Authors:Preibisch, S.; Ejsmont, R.; Rohlfing, T.; Tomancak, P.
Place of Publication:Piscataway
Publisher:IEEE Service Center
Date of Publication (YYYY-MM-DD):2008
Title of Book:2008 IEEE International Symposium on Biomedical Imaging
Start Page:324
End Page:327
Full Name of Book-Editor(s):Institute of Electrical and Electronics Engineers, Engineering in Medicine and Biology Society, Signal processing Society
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Animal development can be described as a complex, threedimensional
cellular system that changes dramatically across
time as a consequence of cell proliferation, differentiation and
movement. Using Drosophila embryogenesis as a model we
are developing molecular, imaging and image analysis techniques
to record an entire developmental system at cellular
resolution.
We image Drosophila embryos expressing fluorescent
markers in toto using Single Plane Illumination Microscopy
(SPIM). SPIM offers the unique ability to image large living
biological specimens in their entirety by acquiring image
stacks from multiple angles while also providing high temporal
resolution necessary for following dynamic developmental
processes. We have developed an image analysis pipeline that
efficiently processes long-term time-lapse multi-view SPIM
data by aligning the different angles with high precision for
a single time point and propagating the alignment parameters
throughout the time series. The registered views are fused
using an approach that evaluates the image content in each
view.
External Publication Status:published
Document Type:InBook
Communicated by:n.n.
Affiliations:MPI f�r molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1147
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