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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2008     Display Documents



ID: 414404.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2008
siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis
Authors:Grimard, Vinciane; Massier, Julia; Richter, Doris; Schwudke, Dominik; Kalaidzidis, Yannis; Fava, Eugenio; Hermetter, Albin; Thiele, Christoph
Date of Publication (YYYY-MM-DD):2008
Title of Journal:Journal of Lipid Research
Volume:49
Issue / Number:11
Start Page:2427
End Page:2440
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Lipid homeostasis is essential for proper function of cells and organisms. To unravel new regulators of this system, we developed a screening procedure, combining RNA interference in HeLa cells and TLC, which enabled us to monitor modifications of lipid composition resulting from short, interfering RNA knock-downs. We applied this technique to the analysis of 600 human kinases. Despite the occurrence of off-target effects, we identified JNK2 as a new player in triglyceride (TG) homeostasis and lipid droplet metabolism and, more specifically, in the regulation of lipolysis. Similar control of the level of TGs and lipid droplets was observed for its Schizosaccharomyces pombe homolog, Sty1, suggesting an evolutionary conserved function of mitogen-activated protein kinases in the regulation of lipid storage in eukaryotic cells.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI f�r molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1155
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