Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2008     Display Documents



ID: 414419.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2008
Ceramide triggers budding of exosome vesicles into multivesicular endosomes
Authors:Trajkovic, Katarina; Hsu, Chieh; Chiantia, Salvatore; Rajendran, Lawrence; Wenzel, Dirk; Wieland, Felix; Schwille, Petra; Brugger, Britta; Simons, Mikael
Date of Publication (YYYY-MM-DD):2008
Title of Journal:Science
Volume:319
Issue / Number:5867
Start Page:1244
End Page:1247
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation.
External Publication Status:published
Document Type:Article
Communicated by:n.n.
Affiliations:MPI f�r molekulare Zellbiologie und Genetik
Identifiers:LOCALID:1177
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.