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          Institute: MPI für molekulare Genetik     Collection: Sequencing Group     Display Documents

ID: 416183.0, MPI für molekulare Genetik / Sequencing Group
Increasing genomic information in bivalves through new EST collections in four species: development of new genetic markers for environmental studies and genome evolution
Authors:Tanguy, Arnaud; Bierne, Nicolas; Saavedra, Carlos; Pina, Benjamin; Bachère, Evelyne; Kube, Michael; Bazin, Eric; Bonhomme, François; Boudry, Pierre; Boulo, Viviane; Boutet, Isabelle; Cancela, Leonor; Dossat, Carole; Favrel, Pascal; Huvet, Arnaud; Jarque, Sergio; Jollivet, Didier; Klages, Sven; Lapègue, Sylvie; Leite, Ricardo; Moal, Jeanne; Moraga, Dario; Reinhardt, Richard; Samain, Jean-François; Zouros, Eleftherios; Canario, Adelino
Date of Publication (YYYY-MM-DD):2008-01-31
Title of Journal:Gene
Journal Abbrev.:Gene
Issue / Number:1-2
Start Page:27
End Page:36
Copyright:© 2007 Elsevier B.V. All rights reserved.
Review Status:not specified
Audience:Experts Only
Abstract / Description:The generation of EST information is an essential step in the genomic characterisation of species. In the context of the European Network Marine Genomics, a common goal was to significantly increase the amount of ESTs in commercial marine mollusk species and more specifically in the less studied but ecologically and commercially important groups, such as mussel and clam genera. Normalized cDNA libraries were constructed for four different relevant bivalves species (Crassostrea gigas, Mytilus edulis, Ruditapes decussatus and Bathymodiolus azoricus), using numerous tissues and physiological conditions. In this paper, we present the analysis of the 13,013 expressed sequence tags (ESTs) generated. Each EST library was independently assembled and 1300–3000 unique sequences were identified in each species. For the different species, functional categories could be assigned to only about 16 to 27% of ESTs using the GO annotation tool. All sequences have been incorporated into a publicly available database and form the basis for subsequent microarray design, SNP detection and polymorphism analysis, and the placement of novel markers on genetic linkage maps.
Free Keywords:Molluscs; ESTs; Environment; Evolution
External Publication Status:published
Document Type:Article
Communicated by:Richard Reinhardt
Affiliations:MPI für molekulare Genetik
External Affiliations:1.UMR CNRS 7144 UPMC Evolution et Génétique des Populations Marines, Station Biologique, Université Européenne de Bretagne, BP 74, Place Georges Teissier, 29682 Roscoff, France;
2.Université de Montpellier II – Institut des Sciences de l'Evolution de Montpellier – Biologie Intégrative – UMR CNRS 5554, Station Méditerranéenne de l'Environnement Littoral, 34200 Sète, France;
3.Instituto de Acuicultura de Torre la Sal (IATS), Consejo Superior de Investigaciones Cientificas (CSIC), 12595 Ribera de Cabanes, Castellon, Spain;
4.IBMB-CSIC, Jordi Girona, 18, 08034 Barcelona, Spain;
5.IFREMER CNRS UM2, GPIA CC 80, 2 Place Eugène Bataillon, 34095 Montpellier cedex5, France
6.Laboratoire de Génétique et Pathologie, IFREMER La Tremblade, 17390 La Tremblade, France;
7.University of Algarve / CCMAR, Campus de Gambelas, 8005-139 Faro, Portugal;
8.GENOSCOPE, 2 rue Gaston Crémieux, CP 5706, 91057 Evry Cedex, France;
9.UMR 100 IFREMER-Université de Caen Basse Normandie, “Physiologie et Ecophysiologie des Mollusques Marins”, - IBFA - 14032 - Caen Cedex – France;
10.UMR 100 Physiologie et Ecophysiologie des Mollusques Marins, IFREMER Centre de Brest, B.P. 70, 29280 Plouzané, France;
11.Laboratoire des Sciences de l'Environnement Marin (LEMAR), UMR-CNRS 6539, Institut Universitaire Européen de la Mer, Université de Bretagne Occidentale, Place Nicolas Copernic, 29280, Plouzané, France;
12.Department of Biology, University of Crete, 71409 Iraklion, Crete, Greece;
13.Centro de Ciencias do Mar, Universidade do Algarve, Campus de Gambelas, 8005 - 139 FARO, Portugal.
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