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          Institute: MPI für Biochemie     Collection: Molecular Medicine (R. Fässler)     Display Documents



ID: 41644.0, MPI für Biochemie / Molecular Medicine (R. Fässler)
Identification and characterization of Pkhd1, the mouse orthologue of the human ARPKD gene
Authors:Nagasawa, Y.; Matthiesen, S.; Onuchic, L. F.; Hou, X. Y.; Bergmann, C.; Esquivel, E.; Senderek, J.; Ren, Z. Y.; Zeltner, R.; Furu, L.; Avner, E.; Moser, M.; Somlo, S.; Guay-Woodford, L.; Buttner, R.; Zerres, K.; Germino, G. G.
Language:English
Date of Publication (YYYY-MM-DD):2002-09
Title of Journal:Journal of the American Society of Nephrology
Journal Abbrev.:J. Am. Soc. Nephrol.
Volume:13
Issue / Number:9
Start Page:2246
End Page:2258
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:PKHD1, the gene mutated in human autosomal recessive polycystic kidney disease has recently been identified. Its translation products are predicted to belong to a superfamily of proteins involved in the regulation of cellular adhesion and repulsion. One notable aspect of the gene is its unusually complex pattern of splicing. This study shows that mouse Pkhd1 and its translation products have very similar properties to its human orthologue. Mouse Pkhd1 extends over approximately 500 kb of genomic DNA, includes a minimum of 68 nonoverlapping exons, and exhibits a complex pattern of splicing. The longest ORF encodes a protein of 4059aa predicted to have an N-terminal signal peptide, multiple IPTs and PbH1 repeats, a single transmembrane span (TM), and a short cytoplasmic C-terminus. Although the protein sequence is generally well conserved (approximately 73% average identity), the C-termini share only 55% identity. The pattern of Pkhd1 expression by in situ hybridization was also examined in developing and adult mouse tissues over a range of ages (E12.5 to 3 mo postnatal). High levels of expression were present in renal and biliary tubular structures at all time points examined. Prominent Pkhd1 signals were also found in a number of other organs and tissues. Tissue-specific differences in transcript expression were revealed through the use of single exon probes. These data show that key features of human PKHD1 are highly conserved in the mouse and suggest that the complicated pattern of splicing is likely to be functionally important.
Comment of the Author/Creator:Date: 2002, SEP
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Molecular Medicine (R. Fässler)/Research Group Moser
External Affiliations:Rhein Westfal TH Aachen, Inst Human Genet, Pauwelsstr 30, D-; 52074 Aachen, Germany; Rhein Westfal TH Aachen, Inst Human Genet, D-52074 Aachen, Germany; Johns Hopkins Univ, Sch Med, Dept Med & Genet, Div Nephrol, Baltimore, MD 21205 USA; Univ Bonn, Inst Pathol, D-5300 Bonn, Germany; Univ Sao Paulo, Dept Med, Sao Paulo, Brazil; Univ Alabama, Dept Med & Pediat, Birmingham, AL USA; Yale Univ, Dept Med & Genet, New Haven, CT USA; Rainbow Babies Children Hosp, Dept Pediat, Cleveland, OH USA
Identifiers:ISI:000177687000007 [ID No:1]
ISSN:1046-6673 [ID No:2]
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