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          Institute: MPI für Biochemie     Collection: Emeriti Groups     Display Documents

ID: 41646.0, MPI für Biochemie / Emeriti Groups
Gene structure and functional analysis of the mouse nidogen-2 gene: Nidogen-2 is not essential for basement membrane formation in mice
Authors:Schymeinsky, J.; Nedbal, S.; Miosge, N.; Poschl, E.; Rao, C.; Beier, D. R.; Skarnes, W. C.; Timpl, R.; Bader, B. L.
Date of Publication (YYYY-MM-DD):2002-10
Title of Journal:Molecular and Cellular Biology
Journal Abbrev.:Mol. Cell. Biol.
Issue / Number:19
Start Page:6820
End Page:6830
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Nidogens are highly conserved proteins in vertebrates and invertebrates and are found in almost all basement membranes. According to the classical hypothesis of basement membrane organization, nidogens connect the laminin and collagen IV networks, so stabilizing the basement membrane, and integrate other proteins. In mammals two nidogen proteins, nidogen-1 and nidogen-2, have been discovered. Nidogen-2 is typically enriched in endothelial basement membranes, whereas nidogen-1 shows broader localization in most basement membranes. Surprisingly, analysis of nidogen-1 gene knockout mice presented evidence that nidogen-1 is not essential for basement membrane formation and may be compensated for by nidogen-2. In order to assess the structure and in vivo function of the nidogen-2 gene in mice, we cloned the gene and determined its structure and chromosomal location. Next we analyzed mice carrying an insertional mutation in the nidogen-2 gene that was generated by the secretory gene trap approach. Our molecular and biochemical characterization identified the mutation as a phenotypic null allele. Nidogen-2-deficient mice show no overt abnormalities and are fertile, and basement membranes appear normal by ultrastructural analysis and immunostaining. Nidogen- 2 deficiency does not lead to hemorrhages in mice as one may have expected. Our results show that nidogen-2 is not essential for basement membrane formation or maintenance.
Comment of the Author/Creator:Date: 2002, OCT
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Emeriti Groups/Protein Chemistry (R. Timpl)
MPI für Biochemie/Molecular Biology (A. Ullrich)/Research Group B. Bader
External Affiliations:Univ Gottingen, Dept Histol, Ctr Anat, D-37075 Gottingen, Germany; Univ Erlangen Nurnberg, Dept Expt Med 1, D-91045 Erlangen, Germany; Harvard Univ, Brigham & Womens Hosp, Sch Med, Boston, MA 02115 USA; Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Identifiers:ISI:000177961900017 [ID No:1]
ISSN:0270-7306 [ID No:2]
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