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          Institute: MPI für Biochemie     Collection: Independent Junior Research Groups     Display Documents

ID: 41655.0, MPI für Biochemie / Independent Junior Research Groups
Transcription factor Sp3 is silenced through SUMO modification by PIAS1
Authors:Sapetschnig, A.; Rischitor, G.; Braun, H.; Doll, A.; Schergaut, M.; Melchior, F.; Suske, G.
Date of Publication (YYYY-MM-DD):2002-10-01
Title of Journal:EMBO Journal
Journal Abbrev.:Embo J.
Issue / Number:19
Start Page:5206
End Page:5215
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Sp3 is a ubiquitous transcription factor closely related to Sp1. Here we show that Sp3 is a target for SUMO modification in vivo and in vitro. SUMO modification of Sp3 occurs at a single lysine located between the second glutamine-rich activation domain and the DNA-binding domain. Mutational analyses identified the sequence IKXE as essential for SUMO conjugation to Spa. We identified the protein inhibitor of activated STAT1 (PIAS1) as an interaction partner of Sp3 and Ubc9. Moreover, PIAS1 strongly stimulated SUMO conjugation to Sp3, thus acting as an E3 ligase for SUMO conjugation to Sp3. All mutations that prevented SUMO modification in vitro strongly enhanced the transcriptional activity of Sp3, showing that SUMO modification silences Sp3 activity. SUMO-modified Sp3 bound to DNA with similar specificity and affinity as unmodified Sp3. However, DNA-bound Sp3 did not act as a substrate for SUMO modification.
Free Keywords:E3 ligase; inhibitory domain; PIAS1; SUMO; transcription factor Spa
Comment of the Author/Creator:Date: 2002, OCT 1
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Independent Junior Research Groups/Protein Modification and Nuclear Transport (F. Melchior)
External Affiliations:Inst Mol Biol & Tumorforsch, Emil Mannkopff Str 2, D-35037; Marburg, Germany; Inst Mol Biol & Tumorforsch, D-35037 Marburg, Germany
Identifiers:ISI:000178502600020 [ID No:1]
ISSN:0261-4189 [ID No:2]
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