Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Quick Search
My eDoc
Session History
Support Wiki
Direct access to
document ID:

          Institute: MPI für Biochemie     Collection: Former and Other Members     Display Documents

ID: 41688.0, MPI für Biochemie / Former and Other Members
Simulated microgravity alters differentiation and increases apoptosis in human follicular thyroid carcinoma cells
Authors:Grimm, D.; Bauer, J.; Kossmehl, P.; Shakibaei, M.; Schonberger, J.; Pickenhahn, H.; Schulze-Tanzil, G.; Vetter, R.; Eilles, C.; Paul, M.; Cogoli, A.
Date of Publication (YYYY-MM-DD):2002-02
Title of Journal:FASEB Journal
Journal Abbrev.:Faseb J.
Issue / Number:2
Start Page:U55
End Page:U81
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:This study focuses on the effects of simulated microgravity (0g) on the human follicular thyroid carcinoma cell line ML-1. Cultured on a three-dimensional clinostat, ML-1 cells formed three-dimensional MCTSs (MCTS diameter: 0.3+/-0.01 mm). After 24 and 48 h of clinorotation, the cells significantly decreased fT3 and fT4 secretion but up-regulated the thyroid-stimulating hormone-receptor expression as well as the production of vimentin, vinculin, and extracellular matrix proteins (collagen I and III, laminin, fibronectin, chondroitin sulfate) compared with controls. Furthermore, ML-1 cells grown on the clinostat showed elevated amounts of the apoptosis-associated Fas protein, of p53, and of bax but showed reduced quantities of bcl-2. In addition, signs of apoptosis became detectable, as assessed by terminal deoxynucleotidyl transferase-mediated dUTP digoxigenin nick end labeling, 4', 6-diamidino-2-phenylindole staining, DNA laddering, and 85-kDa apoptosis-related cleavage fragments. These fragments resulted from enhanced 116-kDa poly(ADP-ribose)polymerase (PARP) activity and apoptosis. These observations suggest that clinorotation elevates intermediate filaments, cell adhesion molecules, and extracellular matrix proteins and simultaneously induces apoptosis in follicular thyroid cancer cells. In conclusion, our experiments could provide a regulatory basis for the finding that astronauts show low thyroid hormone levels after space flight, which may be explained by the increase of apoptosis in thyrocytes as a result of simulated 0g.
Free Keywords:extracellular matrix proteins; hormones; clinorotation; thyroid tumor; programmed cell death
Comment of the Author/Creator:Date: 2002, FEB
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Former and Other Members
External Affiliations:Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin; Pharmacol & Toxicol, Garystr 5, D-14195 Berlin, Germany; Free Univ Berlin, Benjamin Franklin Med Ctr, Inst Clin Pharmacol & Toxicol, D-14195 Berlin, Germany; Free Univ Berlin, Inst Anat, D-14195 Berlin, Germany; Univ Regensburg, Dept Nucl Med, D-8400 Regensburg, Germany; ETH Zurich, Space Biol Grp, Zurich, Switzerland
Identifiers:ISI:000174203700003 [ID No:1]
ISSN:0892-6638 [ID No:2]
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.