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          Institute: MPI für Biochemie     Collection: Cell Biology (E. A. Nigg)     Display Documents

ID: 41750.0, MPI für Biochemie / Cell Biology (E. A. Nigg)
Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation in the cytokinetic process
Authors:Goto, H.; Yasui, Y.; Kawajiri, A.; Nigg, E. A.; Terada, Y.; Tatsuka, M.; Nagata, K.; Inagaki, M.
Date of Publication (YYYY-MM-DD):2003-03-07
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J. Biol. Chem.
Issue / Number:10
Start Page:8526
End Page:8530
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Aurora-B is an evolutionally conserved protein kinase that regulates several mitotic events including cytokinesis. We previously demonstrated the possible existence of a protein kinase that phosphorylates at least Ser-72 on vimentin, the most widely expressed intermediate filament protein, in the cleavage furrow-specific manner. Here we showed that vimentin- Ser-72 phosphorylation occurred specifically at the border of the Aurora-B-localized area from anaphase to telophase. Expression of a dominant-negative mutant of Aurora-B led to a reduction of this vimentin-Ser-72 phosphorylation. In vitro analyses revealed that Aurora-B phosphorylates vimentin at similar to2 mol phosphate/mol of substrate for 30 min and that this phosphorylation dramatically inhibits vimentin filament formation. We further identified eight Aurora-B phosphorylation sites, including Ser-72 on vimentin, and then constructed the mutant vimentin in which these identified sites are changed into Ala. Cells expressing this mutant formed an unusually long bridge-like intermediate filament structure between unseparated daughter cells. We then identified important phosphorylation sites for the bridge phenotype. Our findings indicate that Aurora-B regulates the cleavage furrow-specific vimentin phosphorylation and controls vimentin filament segregation in cytokinetic process.
Comment of the Author/Creator:Date: 2003, MAR 7
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Cell Biology (E.A. Nigg)
External Affiliations:Aichi Canc Ctr, Res Inst, Div Biochem, Chikusa Ku, Nagoya,; Aichi 4648681, Japan; Aichi Canc Ctr, Res Inst, Div Biochem, Chikusa Ku, Nagoya, Aichi 4648681, Japan; Nagoya Univ, Sch Med, Dept Pathol, Nagoya, Aichi 4668550, Japan; Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN 55455 USA; Hiroshima Univ, Res Inst Radiat Biol & Med, Hiroshima 7348553, Japan
Identifiers:ISI:000181466800101 [ID No:1]
ISSN:0021-9258 [ID No:2]
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