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          Institute: MPI für Biochemie     Collection: Emeriti Groups     Display Documents



ID: 41766.0, MPI für Biochemie / Emeriti Groups
Tubular-vesicular transformation in the contractile vacuole system of Dictyostelium
Authors:Gerisch, G.; Heuser, J.; Clarke, M.
Language:English
Date of Publication (YYYY-MM-DD):2002
Title of Journal:Cell Biology International
Journal Abbrev.:Cell Biol. Int.
Volume:26
Issue / Number:10
Start Page:845
End Page:852
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:The contractile vacuole complex of Dictyostelium is the paradigm of a membrane system that undergoes tubular-vesicular transitions during its regular cycle of activities. This system acts as an osmoregulatory organelle in freshwater amoebae and protozoa. It collects fluid in a network of tubules and cisternae, and pumps it out of the cell through transient pores in the plasma membrane. Tubules and vacuoles are interconvertible. The tubular channels are associated with the cortical actin network and are capable of moving and fusing. The contractile vacuole complex is separate from vesicles of the endosomal pathway and preserves its identity in a dispersed state during cell division. We outline techniques to visualize the contractile vacuole system by electron and light microscopy. Emphasis is placed on GFP-fusion proteins that allow visualization of the dynamics of the contractile vacuole network in living cells. Proteins that control activities of this specialized organelle in Dictyostelium have been conserved during evolution and also regulate membrane trafficking in man. (C) 2002 Elsevier Science Ltd. All rights reserved.
Free Keywords:contractile vacuole; Dictyostelium; membrane trafficking; osmoregulation; rab GTPase; vacuolar H+-ATPase
Comment of the Author/Creator:Date: 2002
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Emeriti Groups/Cell Dynamics (G. Gerisch)
External Affiliations:Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA; Oklahoma Med Res Fdn, Program Mol & Cell Biol, Oklahoma City, OK 73104 USA
Identifiers:ISI:000179428900002 [ID No:1]
ISSN:1065-6995 [ID No:2]
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