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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents



ID: 420245.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor
Translation of Title:Single−spike detection in vitro and in vivo with a genetic Ca2+ sensor
Authors:Haydon−Wallace, Damian J.; Meyer zum Alten Borgloh, Stephan; Astori, Simone; Yang, Ying; Bausen, Melanie; Kügler, Sebastian; Palmer, Amy E.; Tsien, Roger Y.; Sprengel, Rolf; Kerr, Jason; Denk, Winfried; Hasan, Mazahir
Language:English
Date of Publication (YYYY-MM-DD):2008-08-10
Title of Journal:Nature Methods
Journal Abbrev.:Nature Methods
Volume:5
Start Page:797
End Page:804
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Measurement of population activity with single−action−potential, single−neuron resolution is pivotal for understanding information representation and processing in the brain and how the brain&#39;s responses are altered by experience. Genetically encoded indicators of neuronal activity allow long−term, cell type−specific expression. Fluorescent Ca2+ indicator proteins (FCIPs), a main class of reporters of neural activity, initially suffered, in particular, from an inability to report single action potentials in vivo. Although suboptimal Ca2+−binding dynamics and Ca2+−induced fluorescence changes in FCIPs are important factors, low levels of expression also seem to play a role. Here we report that delivering D3cpv, an improved fluorescent resonance energy transfer−based FCIP, using a recombinant adeno−associated virus results in expression sufficient to detect the Ca2+ transients that accompany single action potentials. In upper−layer cortical neurons, we were able to detect transients associated with single action potentials firing at rates of <1 Hz, with high reliability, from in vivo recordings in living mice.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI f�r medizinische Forschung/Nachwuchsgruppe Neurophysiologie des Verhaltens
MPI f�r medizinische Forschung/Abteilung Biomedizinische Optik
MPI f�r medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI f�r medizinische Forschung/Abteilung Zellphysiologie
Identifiers:LOCALID:7240
URI:http%3A%2F%2Fwww.nature.com%2Fnmeth%2Fjournal%2Fv5...
URI:http%3A%2F%2Fwww.nature.com%2Fnmeth%2Fjournal%2Fv5...
URI:http%3A%2F%2Fwww.nature.com%2Fnmeth%2Fjournal%2Fv5...
DOI:10.1038%2FNMETH.1242
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