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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Plant Developmental Biology (George Coupland)     Display Documents

ID: 42411.0, MPI für Pflanzenzüchtungsforschung / Dept. Plant Developmental Biology (George Coupland)
Soybean ENOD40 encodes two peptides that bind to sucrose synthase
Authors:Röhrig, H.; Schmidt, J.; Miklashevichs, E.; Schell, J.; John, M.
Date of Publication (YYYY-MM-DD):2002-02-19
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Issue / Number:4
Start Page:1915
End Page:1920
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:ENOD40 is expressed at an early stage in root nodule organogenesis in legumes. Identification of ENOD40 homologs in nonleguminous plants suggests that this gene may have a more general biological function. In vitro translation of soybean ENOD40 mRNA in wheat germ extracts revealed that the conserved nucleotide sequence at the 5' end (region 1) encodes two peptides of 12 and 24 aa residues (peptides A and B). These peptides are synthesized de novo from very short, overlapping ORFs. Appropriate ORFs are present in all legume ENOD40s studied thus far. In this case small peptides are directly translated from polycistronic eukaryotic mRNA. The 24-aa peptide B was detected in nodules by Western blotting. Both peptides specifically bind to the same 93-kDa protein, which was affinity purified from soybean nodules. Using pepticle mass fingerprinting, we identified this binding protein as nodulin 100, which is a subunit of sucrose synthase. Based on our data we suggest that ENOD40 peptides are involved in the control of sucrose use in nitrogen-fixing nodules.
Free Keywords:translation; short open reading frames; peptide signals
Comment of the Author/Creator:Date: 2002, FEB 19
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Max Planck Inst Zuchtungsforsch, Carl Von Linne Weg 10, D-50829; Cologne, Germany; Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany
Identifiers:ISI:000174031100032 [ID No:1]
ISSN:0027-8424 [ID No:2]
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