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          Institute: MPI für experimentelle Medizin     Collection: Neurogenetics     Display Documents



ID: 42727.0, MPI für experimentelle Medizin / Neurogenetics
The proteoglycan NG2 is complexed with alpha-amino-3-hydroxy-5- methyl-4-isoxazolepropionic acid (AMPA) receptors by the PDZ glutamate receptor interaction protein (GRIP) in glial progenitor cells - Implications for glial-neuronal signaling
Authors:Stegmüller, Judith; Werner, Hauke; Nave, Klaus-Armin; Trotter, Jacqueline
Language:English
Date of Publication (YYYY-MM-DD):2003-02-07
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J. Biol. Chem.
Volume:278
Issue / Number:6
Start Page:3590
End Page:3598
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The proteoglycan NG2 is expressed by immature glial cells in the developing and adult central nervous system. Using the COOH-terminal region of NG2 as bait in a yeast two-hybrid screen, we identified the glutamate receptor interaction protein GRIP1, a multi-PDZ domain protein, as an interacting partner. NG2 exhibits a PDZ binding motif at the extreme COOH terminus which binds to the seventh PDZ domain of GRIPl. In addition to the published expression in neurons, GRIPl is expressed by immature glial cells. GRIPl is known to bind to the GluRB subunit of the AMPA glutamate receptor expressed by subpopulations of neurons and immature glial cells. In cultures of primary oligodendrocytes, cells coexpress GluRB and NG2. A complex of NG2, GRIP1, and GluRB can be precipitated from transfected mammalian cells and from cultures of primary oligodendrocytes. Furthermore, NG2 and GRIP can be coprecipitated from developing brain tissue. These data suggest that GRIPl acts as a scaffolding molecule clustering NG2 and AMPA receptors in immature glia. In view of the presence of synaptic contacts between neurons and NG2-positive glial cells in the hippocampus and the close association of NG2-expressing glial cells with axons, we suggest a role for the NG2-AMPA receptor complex in glial-neuronal recognition and signaling.
Comment of the Author/Creator:Date: 2003, FEB 7
External Publication Status:published
Document Type:Article
Communicated by:Klaus-Armin Nave
Affiliations:MPI für experimentelle Medizin/Neurogenetics
External Affiliations:Univ Heidelberg, Dept Neurobiol, Neuenheimer Feld 364, D-69120; Heidelberg, Germany; Univ Heidelberg, Dept Neurobiol, D-69120 Heidelberg, Germany; Univ Mainz, Dept Zool, Unit Mol Cell Biol, D-55099 Mainz, Germany
Identifiers:ISI:000180869700012 [ID No:1]
ISSN:0021-9258 [ID No:2]
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